DAZ-associated protein 1 (DAZAP1)
The protein contains 407 amino acids for an estimated molecular weight of 43383 Da.
RNA-binding protein, which may be required during spermatogenesis. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| a breast cancer sample; somatic mutation |
Biological Process
Cell differentiation
Cell population proliferation
Maternal placenta development
Positive regulation of mRNA splicing, via spliceosome
Regulation of RNA metabolic process
Spermatogenesis
Cellular Component
Cytoplasm
Cytosol
Nucleoplasm
Nucleus
Protein complex
Protein-containing complex
Ribonucleoprotein complex
The reference OMIM entry for this protein is 607430
Daz-associated protein 1; dazap1
CLONING
Using a yeast 2-hybrid screen of a human testis cDNA library with the coding region of DAZ (400003) as bait, Tsui et al. (2000) cloned cDNAs encoding DAZAP1 and DAZAP2 (607431). DAZAP1 is an RNA-binding protein of 407 amino acids. It contains 2 RNP motifs in the N terminus and has a proline-rich C terminus. The protein shares high sequence homology in the RNP regions with hnRNP A/B proteins (see 600124), but differs from these proteins in the C terminus. Northern blot analysis detected expression of 2 DAZAP1 transcripts of 2.75 and 2.4 kb that were most abundant in the testis and to a lesser degree in the thymus. All other tissues expressed low levels of the transcript. RT-PCR of RNA samples from testicular biopsies of 4 infertile men detected expression of DAZAP1 only in samples with normal spermatogenesis, suggesting that DAZAP1 is expressed mainly in the germ cells.GENE FUNCTION
Using GST-fusion binding analysis, Tsui et al. (2000) determined that both DAZAP1 and DAZAP2 interact with DAZ and DAZL (601486). Deletion constructs indicated that DAZ and DAZL bind to DAZAP1/DAZAP2 mainly through the DAZ repeat region.MAPPING
By FISH, Tsui et al. (2000) mapped the DAZAP1 gene to chromosome 19p13.3. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 607430 was added.
Feb. 24, 2016: Protein entry updated
Automatic update: model status changed