General vesicular transport factor p115 (USO1)
The protein contains 962 amino acids for an estimated molecular weight of 107895 Da.
General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity. (updated: Oct. 25, 2017)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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Biological Process
COPII vesicle coating
Endoplasmic reticulum to Golgi vesicle-mediated transport
Golgi organization
Golgi vesicle docking
Intracellular protein transport
Membrane fusion
Mitotic cell cycle
Regulation of cellular response to insulin stimulus
Secretory granule localization
Small GTPase mediated signal transduction
Transcytosis
Vesicle fusion with Golgi apparatus
Cellular Component
Cytosol
Endoplasmic reticulum
ER to Golgi transport vesicle membrane
Fibrillar center
Golgi apparatus
Golgi membrane
Golgi stack
Membrane
Microtubule organizing center
Nucleolus
Perinuclear region of cytoplasm
Transport vesicle
Molecular Function
Cadherin binding
Obsolete protein transporter activity
Poly(A) RNA binding
RNA binding
The reference OMIM entry for this protein is 603344
Uso1 vesicle docking protein, s. cerevisiae, homolog of; uso1
Vesicle docking protein, 115-kd; p115
Transcytosis-associated protein; tap
Tap/p115
CLONING
Vesicular transport of proteins is carried out by the formation of coated vesicles from a donor compartment, followed by their uncoating and subsequent docking and fusion of the vesicles with a target compartment membrane. Waters et al. (1992) identified a 115-kD bovine liver protein, designated p115, that is required for transport from the cis to the medial compartments of the Golgi apparatus. Barroso et al. (1995) found that p115 is identical to rat liver TAP (transcytosis-associated protein), a protein found on transcytotic vesicles and required for their fusion with the target membrane. TAP/p115 is homologous to Usop1, an S. cerevisiae protein involved in endoplasmic reticulum to Golgi transport. The authors suggested that TAP/p115/Usop1 is a general factor acting within the secretory and endocytic pathways to bind transport vesicles prior to membrane fusion. By screening a liver library with a rat p115 cDNA, Sohda et al. (1998) isolated a cDNA encoding human p115. The predicted 962-amino acid human p115 is 95% and 92% identical to bovine and rat p115, respectively.GENE FUNCTION
Sohda et al. (1998) stated that p115 is recycled between the cytosol and the Golgi apparatus during interphase. They demonstrated that the membrane interaction of p115 is regulated by phosphorylation: dephosphorylated p115 associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Allan et al. (2000) demonstrated that the tethering factor p115 is a RAB1 (179508) effector that binds directly to activated RAB1. RAB1 recruited p115 to coat protein complex II (COPII; see 601924) vesicles during budding from the endoplasmic reticulum, where it interacted with a select set of COPII vesicle-associated SNAREs (see 603215) to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Allan et al. (2000) proposed that RAB1-regulated assembly of functional effector-SNARE complexes defines a conserved molecular mechanism to coordinate recognition between subcellular compartments.MAPPING
Gross (2012) mapped the USO1 gene to chromosome 4q21.1 based on an alignment of a USO1 sequence (GenBank GENBANK BC006398) with the genomic sequence (GRCh37). ... More on the omim web site
Feb. 10, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 603344 was added.