Protein DPCD (DPCD)
The protein contains 203 amino acids for an estimated molecular weight of 23240 Da.
May play a role in the formation or function of ciliated cells. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs1331419018 | |
| dbSNP:rs7006 |
No binding partner found
Biological Process
Determination of left/right symmetry
Epithelial cilium movement involved in extracellular fluid movement
Flagellated sperm motility
Lateral ventricle development
Left/right pattern formation
Spermatogenesis
Third ventricle development
Molecular Function
The reference OMIM entry for this protein is 616467
Deleted in primary ciliary dyskinesia, mouse, homolog of; dpcd
CLONING
By searching databases for homologs of mouse Dpcd, followed by RT-PCR of cultured human bronchial epithelial (HBE) cells, Zariwala et al. (2004) cloned human DPCD. The predicted 23-kD human DPCD protein shares 88% identity with the mouse protein. It contains potential phosphorylation, glycosylation, and amidation sites. Like mouse Dpcd, human DPCD overlaps the POLL gene (606343) on the opposite strand. Northern blot analysis of human tissues revealed highest expression of a 0.82-kb DPCD transcript in testis, similar to POLL. Much lower DPCD expression was detected in pancreas, skeletal muscle, and heart.MAPPING
Zariwala et al. (2004) reported that human DPCD maps to chromosome 10, where it partially overlaps the POLL gene on the opposite strand. The mouse Dpcd gene is located in the syntenic region on chromosome 19. Gross (2015) mapped the DPCD gene to chromosome 10q24.32 based on an alignment of the DPCD sequence (GenBank GENBANK BC031695) with the genomic sequence (GRCh38).ANIMAL MODEL
Kobayashi et al. (2002) generated Poll -/- mice, which exhibited hydrocephalus, high mortality rate after birth, situs inversus totalis, chronic sinusitis, and male infertility due to immotility of sperm. Consistent with the phenotype, ultrastructural analysis revealed a defect of inner dynein arms in cilia from ependymal cells and respiratory epithelium of Poll -/- mice. Zariwala et al. (2004) examined the genetic construct used by Kobayashi et al. (2002) to generate Poll -/- mice, which exhibited features of primary ciliary dyskinesia (PCD; 244400). They noted that deletion of only the catalytic domain of Poll in a separate study by Bertocci et al. (2002) resulted in mice with a normal phenotype. Zariwala et al. (2004) determined that deletion of Poll by Kobayashi et al. (2002) also deleted exon 1 of Dpcd on the opposite strand. Northern blot analysis of cultured HBE cells showed that DPCD expression increased during ciliated cell differentiation, whereas expression of POLL decreased. Zariwala et al. (2004) concluded that the PCD phenotype observed in the Poll -/- mice reported by Kobayashi et al. (2002) likely resulted from loss of Dpcd rather than Poll. ... More on the omim web site
May 12, 2019: Protein entry updated
Automatic update: model status changed
Nov. 17, 2018: Protein entry updated
Automatic update: model status changed
Feb. 10, 2018: Protein entry updated
Automatic update: OMIM entry 616467 was added.
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Oct. 27, 2017: Protein entry updated
Automatic update: model status changed
Feb. 25, 2016: Protein entry updated
Automatic update: model status changed
Feb. 24, 2016: Protein entry updated
Automatic update: model status changed
Jan. 25, 2016: Protein entry updated
Automatic update: model status changed