NIF3-like protein 1 (NIF3L1)
The protein contains 377 amino acids for an estimated molecular weight of 41968 Da.
May function as a transcriptional corepressor through its interaction with COPS2, negatively regulating the expression of genes involved in neuronal differentiation. (updated: Oct. 25, 2017)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs7917 |
The reference OMIM entry for this protein is 605778
Nif3-like 1; nif3l1
Als2 chromosome region gene 1; als2cr1
CLONING
Using a fragment isolated by suppression subtractive hybridization from 2 male germ cell lines to screen an adult mouse testis cDNA library, Tascou et al. (2000) identified a novel cDNA, which they termed Nif3l1 because it shows high homology to the S. pombe Ngg1-interacting factor-3 homolog. They isolated human NIF3L1 by EST database searching and sequencing of clones derived from neuroepithelium and brain cDNA libraries. The mouse and human cDNAs encode deduced proteins of 376 and 377 amino acids, respectively, that share 84% sequence identity. Both proteins show strong homology to proteins of lower species, indicating a high degree of conservation from bacteria to mammals. Northern blot analysis showed ubiquitous expression of mouse Nif3l1, with a major transcript size of 1.85 kb. RT-PCR analysis demonstrated ubiquitous expression of Nif3l1 throughout mouse embryonic development. Expression analysis on cell lines revealed strong overexpression in a spermatogonia-derived cell line and a teratocarcinoma cell line. A human NIF3L1/GFP fusion protein showed localization of the protein in the cytoplasm. By sequencing candidate genes in the ALS2 (205100) critical region, Hadano et al. (2001) identified NIF3L1, which they designated ALS2CR1. The deduced 350-amino acid protein has a calculated molecular mass of about 39 kD. Northern blot analysis detected a major 1.6-kb transcript and a minor 4.2-kb transcript that were expressed at highest levels in heart, skeletal muscle, and testis.GENE STRUCTURE
Tascou et al. (2000) determined that the NIF3L1 gene contains 7 exons and spans 14.5 kb of genomic DNA.MAPPING
By STS analysis and electronic PCR, Tascou et al. (2000) mapped the NIF3L1 gene to chromosome 2q33. By FISH, they mapped the mouse Nif3l1 gene to a region of conserved synteny on chromosome 1C. ... More on the omim web site
Feb. 10, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 605778 was added.