CTP synthase 2 (CTPS2)
The protein contains 586 amino acids for an estimated molecular weight of 65678 Da.
Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Constitutes the rate-limiting enzyme in the synthesis of cytosine nucleotides. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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Biological Process
'de novo' CTP biosynthetic process
CTP biosynthetic process
Glutamine metabolic process
Nucleobase-containing small molecule interconversion
Nucleobase-containing small molecule metabolic process
Pyrimidine nucleobase biosynthetic process
Pyrimidine nucleotide metabolic process
Small molecule metabolic process
The reference OMIM entry for this protein is 300380
Cytidine 5-prime triphosphate synthetase 2; ctps2
Ctp synthetase 2
Ctp synthase 2
DESCRIPTION
CTP synthetase (EC 6.3.4.2) catalyzes the formation of CTP from UTP with the concomitant deamination of glutamine to glutamate. CTP synthetase is the rate-limiting enzyme in the synthesis of cytosine nucleotides, which play an important role in various metabolic processes and provide the precursors necessary for the synthesis of RNA and DNA. Cancer cells that exhibit increased cell proliferation also exhibit an increased activity of CTP synthetase. Thus, CTP synthetase is an attractive target for selective chemotherapy (van Kuilenburg et al., 2000).CLONING
Van Kuilenburg et al. (2000) screened an EST database with the CTPS (123860) sequence and identified CTPS2. Using PCR and RACE with liver cDNA, they cloned a full-length CTPS2 cDNA predicted to encode a 586-amino acid protein that shares 74% amino acid identity with CTPS.GENE FUNCTION
Van Kuilenburg et al. (2000) demonstrated that CTPS2 can function as a CTP synthetase by successfully complementing the phenotype of a cytidine-requiring CTP synthetase-deficient mutant in E. coli.MAPPING
By genomic sequence analysis, van Kuilenburg et al. (2000) mapped the CTPS2 gene to chromosome Xp22. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
June 20, 2017: Protein entry updated
Automatic update: comparative model was added.
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 300380 was added.
Feb. 24, 2016: Protein entry updated
Automatic update: model status changed