14 kDa phosphohistidine phosphatase (PHPT1)
The protein contains 125 amino acids for an estimated molecular weight of 13833 Da.
Exhibits phosphohistidine phosphatase activity. (updated: April 1, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology.
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Biological Process
Negative regulation of ATP citrate synthase activity
Negative regulation of lyase activity
Negative regulation of T cell receptor signaling pathway
Peptidyl-histidine dephosphorylation
Positive regulation of cell motility
Positive regulation of insulin secretion involved in cellular response to glucose stimulus
Protein dephosphorylation
Regulation of actin cytoskeleton reorganization
The reference OMIM entry for this protein is 610167
Phosphohistidine phosphatase 1; phpt1
Sex-regulated protein janus-a
DESCRIPTION
PHPT1 is an EDTA-insensitive phosphohistidine phosphatase that catalyzes the dephosphorylation of phosphopeptide I (Ek et al., 2002).CLONING
By database analysis with partial peptide sequences of a phosphohistidine phosphatase purified from porcine liver cytosol as probe, Ek et al. (2002) identified a homologous human sequence from adrenal gland. Using PCR techniques, they cloned a full-length human PHPT1 cDNA from embryonic kidney (HEK293) cells. The deduced 125-amino acid PHPT1 protein migrated as a single, approximately 14 kD band by SDS-PAGE. Northern blot analysis detected a 0.6-kb transcript, with highest expression in heart and skeletal muscle and lower expression in several other tissues including pancreas, liver, and kidney.GENE FUNCTION
Ek et al. (2002) found that recombinant PHPT1 overexpressed and purified from bacteria displayed specific phosphohistidine phosphatase activity toward phosphopeptide I. No activity was detected towards phosphoserine, phosphothreonine, or phosphotyrosine peptides.GENE STRUCTURE
Ek et al. (2002) determined that the PHPT1 gene contains 3 exons.MAPPING
By sequence analysis, Ek et al. (2002) mapped the PHPT1 gene to chromosome 9q34.3. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 15, 2016: Protein entry updated
Automatic update: OMIM entry 610167 was added.
Jan. 27, 2016: Protein entry updated
Automatic update: model status changed
Jan. 24, 2016: Protein entry updated
Automatic update: model status changed