Obg-like ATPase 1 (OLA1)
The protein contains 396 amino acids for an estimated molecular weight of 44744 Da.
Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP. (updated: April 1, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| a breast cancer sample; somatic mutation |
The reference OMIM entry for this protein is 611175
Obg-like atpase 1; ola1
Gtp-binding protein 9; gtpbp9
CLONING
Koller-Eichhorn et al. (2007) identified human GTPBP9, which they called OLA1, as the homolog of the E. coli P-loop NTPase YchF, a member of the Obg-related family of GTPases belonging to the TRAFAC (translation factors) class. The deduced 396-amino acid protein consists of an N-terminal G domain, flanked on either side by an inserted coiled-coil, and a C-terminal TGS domain.GENE FUNCTION
Koller-Eichhorn et al. (2007) demonstrated that OLA1 binds and hydrolyzes ATP more efficiently than GTP and thus is a member of a new subclass of NTPases in the Obg family of GTPases. They showed that members of this protein family from different species also have ATPase activity and are not exclusively GTPases. By performing x-ray crystallography with AMPPCP, a nonhydrolyzable analog of ATP, Koller-Eichhorn et al. (2007) found that one cause of the altered nucleotide specificity of this subclass of NTPases is a substitution of a hydrophobic amino acid for a glutamine in all members of the Obg family. They noted that the same substitution inactivates Ras-like GTPases.MAPPING
Scott (2007) mapped the GTPBP9 gene to chromosome 2q31.1 based on an alignment of the GTPBP9 sequence (GenBank GENBANK AC013467) with the genomic sequence (build 36.2). ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 611175 was added.
Jan. 28, 2016: Protein entry updated
Automatic update: model status changed
Jan. 24, 2016: Protein entry updated
Automatic update: model status changed