THUMP domain-containing protein 1 (THUMPD1)
The protein contains 353 amino acids for an estimated molecular weight of 39315 Da.
Functions as a tRNA-binding adapter to mediate NAT10-dependent tRNA acetylation (PubMed:25653167). (updated: Sept. 27, 2017)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs11074471 |
The reference OMIM entry for this protein is 616662
Thump domain-containing protein 1; thumpd1
Tan1, yeast, homolog of
DESCRIPTION
THUMPD1 functions as an adaptor protein for secondary modification of transfer RNA (tRNA) by cytosine acetylation (Sharma et al., 2015).CLONING
Sharma et al.. (2015) reported that the deduced 353-amino acid THUMPD1 protein has a conserved tRNA-binding THUMP domain between amino acids 111 and 254.GENE FUNCTION
Sharma et al. (2015) found that overexpression of human NAT10 (609221) restored tRNA acetylation in yeast lacking the tRNA adaptor protein Tan1, the ortholog of human THUMPD1. Knockdown of NAT10 reduced tRNA acetylation in HCT116 cells. Coprecipitation analysis of HCT116 cells revealed that NAT10 interacted directly with THUMPD1. NAT10 also acetylated cytosines in 18S ribosomal RNA (see 180450) in a THUMPD1-independent manner. Sharma et al. (2015) proposed that THUMPD1 functions like Tan1 as a specific adaptor to mediate NAT10-dependent tRNA acetylation.MAPPING
Hartz (2015) mapped the THUMPD1 gene to chromosome 16p12.3 based on an alignment of the THUMPD1 sequence (GenBank GENBANK AK000281) with the genomic sequence (GRCh38). ... More on the omim web site
Feb. 10, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 10, 2018: Protein entry updated
Automatic update: OMIM entry 616662 was added.
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Feb. 24, 2016: Protein entry updated
Automatic update: model status changed