Peptidyl-prolyl cis-trans isomerase-like 1 (PPIL1)
The protein contains 166 amino acids for an estimated molecular weight of 18237 Da.
Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11991638, PubMed:28502770, PubMed:28076346). PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides (PubMed:16595688). (updated: March 28, 2018)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
(right-click above to access to more options from the contextual menu)
| Variant | Description |
|---|---|
| dbSNP:rs12194408 |
Biological Process
MRNA splicing, via spliceosome
Protein folding
Protein peptidyl-prolyl isomerization
The reference OMIM entry for this protein is 601301
Peptidyl-prolyl isomerase-like 1; ppil1
Cyclophilin-related gene 1; cypl1
Cyclophilin (see 123840), first identified as a protein with high binding affinity for the immunosuppressive agent cyclosporin A, is one of the most effective therapeutic agents for prevention of graft rejection after organ transplantation.
CLONING
Ozaki et al. (1996) isolated a human cDNA clone encoding a protein homologous to cyclophilins and showed that it is conserved in species ranging from human to prokaryotes. This cDNA contained an open reading frame of 498 nucleotides encoding a polypeptide of 166 amino acids. The predicted amino acid sequence had 41.6% homology to the human cyclophilins. Northern blot analysis indicated ubiquitous expression in adult human tissues, with the most abundant expression in heart and skeletal muscle.MAPPING
Ozaki et al. (1996) localized the PPIL1 gene to 2p23.3-p23.1 by FISH. However, Mann et al. (1998) assigned the PPIL1 gene to 6p21.1 by FISH and radiation hybrid mapping. ... More on the omim web site
April 12, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 601301 was added.
Jan. 28, 2016: Protein entry updated
Automatic update: model status changed
Jan. 25, 2016: Protein entry updated
Automatic update: model status changed