Disheveled-associated activator of morphogenesis 1 (DAAM1)
The protein contains 1078 amino acids for an estimated molecular weight of 123473 Da.
Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments. Involved in building functional cilia (PubMed:16630611, PubMed:17482208). Involved in the organization of the subapical actin network in multiciliated epithelial cells (By similarity). Together with DAAM2, required for myocardial maturation and sarcomere assembly (By similarity). (updated: Feb. 28, 2018)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology.
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No binding partner found
Biological Process
Actin cytoskeleton organization
Wnt signaling pathway, planar cell polarity pathway
Cellular Component
Ciliary basal body
Cytoplasm
Cytosol
Membrane
Motile cilium
Plasma membrane
Stress fiber
The reference OMIM entry for this protein is 606626
Dishevelled-associated activator of morphogenesis 1; daam1
Kiaa0666
CLONING
By screening size-fractionated brain cDNA libraries for cDNAs with the potential to encode proteins larger than 50 kD, Ishikawa et al. (1998) identified a cDNA which they designated KIAA0666. KIAA0666 encodes a 1,085-amino acid protein predicted to function in cell division. It is 68% identical to the KIAA0381 protein (DAAM2; 606627). RT-PCR analysis detected expression of KIAA0666 in all tissues tested. Habas et al. (2001) identified a novel formin (see FMN1; 136535) homology (FH) protein that they named DAAM1. DAAM1 is a widely expressed protein that contains 1,078 amino acids and is identical to the KIAA0666 protein. Like other FH proteins, DAAM1 contains a central proline-rich FH1 domain and a more C-terminal FH2 domain.GENE FUNCTION
Wnt (see 164975) signaling via the frizzled receptor (Fz; see 600667) controls cell polarity and movement during development. Habas et al. (2001) reported that in human cells and during Xenopus embryogenesis, Wnt/Fz signaling activated the small GTPase Rho (165390), a key regulator of cytoskeleton architecture. Wnt/Fz activation of Rho required the cytoplasmic protein dishevelled (DVL; see 601365) and DAAM1. DAAM1 bound to both DVL and Rho and mediated Wnt-induced DVL-Rho complex formation. Inhibition or depletion of DAAM1 prevented Wnt/Fz activation of Rho and of Xenopus gastrulation, but did not prevent activation of beta-catenin (116806) signaling. Liu et al. (2008) showed that human DAAM1 was autoinhibited by an intramolecular interaction between its N-terminal GTPase domain, which includes an autoinhibitory domain, and its C-terminal autoregulatory domain. Interaction of DAAM1 with DVL disrupted this autoinhibitory interaction, resulting in DAAM1 activation. Mutations within or removal of the autoregulatory domain converted DAAM1 into an active protein that could induce Rho activation. Liu et al. (2008) also demonstrated that Dvl synergized with Daam1 to regulate gastrulation during Xenopus embryogenesis.MAPPING
By radiation hybrid analysis, Ishikawa et al. (1998) mapped the DAAM1 gene to chromosome 14. ... More on the omim web site
April 12, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 10, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 606626 was added.
Feb. 24, 2016: Protein entry updated
Automatic update: model status changed