Sorting nexin-8 (SNX8)
The protein contains 465 amino acids for an estimated molecular weight of 52569 Da.
May be involved in several stages of intracellular trafficking. May play a role in intracellular protein transport from early endosomes to the trans-Golgi network. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| a colorectal cancer sample; somatic mutation |
Biological Process
Early endosome to Golgi transport
Endocytosis
Intracellular protein transport
Vesicle organization
The reference OMIM entry for this protein is 614905
Sorting nexin 8; snx8
DESCRIPTION
SNX8 belongs to a large family of proteins characterized by the presence of an SNX-type phox (see 608512) homology (PX) domain with conserved arg-arg-tyr-X-asp/glu and pro-pro-X-pro-X-lys motifs. SNX family members are predicted to be involved in the sorting of endosomes (Teasdale et al., 2001).CLONING
By searching databases for sequences similar to yeast Snx1 (601272), Teasdale et al. (2001) identified human SNX8. The deduced protein has a 171-amino acid SNX-PH domain in its N-terminal half and a coiled-coil domain in its C-terminal half. Using confocal microscopy, Dyve et al. (2009) found that fluorescence-tagged SNX8 partly colocalized with the endosome marker EEA1 (605070) in punctate vesicular structures in transfected HeLa cells. It also partly colocalized with the retromer components SNX1, VPS35 (601501), SNX2 (605929), and VPS26 (VPS26A; 605506).GENE FUNCTION
By knockdown of SNX8 in HeLa cells, Dyve et al. (2009) determined that SNX8 regulates endosome-to-Golgi transport of Shiga toxin and the plant toxin ricin, possibly via 2 different mechanisms. Knockdown of SNX8 increased Shiga toxin transport to the Golgi, but it moderately reduced ricin transport. Knockdown of SNX8 had no effect on endocytosis of Shiga toxin or ricin. Overexpression of fluorescence-tagged SNX8 resulted in a change in morphology of SNX8-positive structures from small puncta to enlarged vesicles or compartments. Inhibition of PI3 kinase (see 601232) resulted in loss of punctate SNX8 localization. Dyve et al. (2009) concluded that SNX8 is likely a negative regulator of endosome-to-Golgi trafficking.MAPPING
Hartz (2012) mapped the SNX8 gene to chromosome 7p22.3 based on an alignment of the SNX8 sequence (GenBank GENBANK AF121858) with the genomic sequence (GRCh37). ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 614905 was added.