CDP-diacylglycerol--inositol 3-phosphatidyltransferase (CDIPT)
The protein contains 213 amino acids for an estimated molecular weight of 23539 Da.
Catalyzes the biosynthesis of phosphatidylinositol (PtdIns) as well as PtdIns:inositol exchange reaction. May thus act to reduce an excessive cellular PtdIns content. The exchange activity is due to the reverse reaction of PtdIns synthase and is dependent on CMP, which is tightly bound to the enzyme. (updated: Sept. 12, 2018)
Protein identification was indicated in the following studies:
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology, is annotated as membranous in UniProt, is predicted to be membranous by TOPCONS.
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| Variant | Description |
|---|---|
| dbSNP:rs1802002 |
The reference OMIM entry for this protein is 605893
Cdp-diacylglycerol-inositol 3-phosphatidyltransferase; cdipt
Phosphatidylinositol synthase 1; pis1
DESCRIPTION
The breakdown products of phosphatidylinositols (PtdIns) are ubiquitous second messengers downstream of many G protein-coupled receptors and tyrosine kinases involved in mitogenesis, the regulation of calcium metabolism, and protein kinase C (see 176982) activation. There are 2 enzymes in the PtdIns biosynthetic pathway, CDP-diacylglycerol synthase (see CDS1; 603548) and PtdIns synthase (PIS).CLONING
By EST database searching, library screening, and RACE analysis, Lykidis et al. (1997) obtained cDNAs encoding CDS1, CDS2 (603549), and CDIPT, which they called PIS1. The deduced 213-amino acid CDIPT protein is highly conserved in yeast and mammals. Northern blot analysis revealed ubiquitous expression of a 2.1-kb CDIPT transcript, with slightly higher levels in liver and skeletal muscle than in other tissues. Functional analysis showed that expression of CDIPT in COS cells caused an approximately 24-fold increase in PIS activity and in PtdIns:Ins exchange activity. However, overexpression of CDIPT, CDS1, or both did not significantly alter the steady-state amount of PtdIns.MAPPING
Lykidis et al. (1997) mapped the CDIPT gene to chromosome 16 based on its inclusion in mapped clones. ... More on the omim web site
June 30, 2020: Protein entry updated
Automatic update: OMIM entry 605893 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).