60S ribosomal protein L21 (RPL21)
The protein contains 160 amino acids for an estimated molecular weight of 18565 Da.
Component of the large ribosomal subunit. (updated: Jan. 23, 2007)
Protein identification was indicated in the following studies:
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs17085349 | |
| HYPT12 |
Biological Process
Cytoplasmic translation
Nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
RRNA processing
SRP-dependent cotranslational protein targeting to membrane
Translation
Translational initiation
Viral transcription
Cellular Component
Cytosol
Cytosolic large ribosomal subunit
Cytosolic ribosome
Endoplasmic reticulum
Membrane
The reference OMIM entry for this protein is 603636
Ribosomal protein l21; rpl21
CLONING
By searching sequence databases with the partial sequences of randomly selected cDNAs from a human colorectal cDNA library, Frigerio et al. (1995) identified cDNAs encoding homologs of rat ribosomal proteins S5 (RPS5; 603630), S9 (RPS9; 603631), S10 (RPS10; 603632), S29 (RPS29; 603633), L5 (RPL5; 603634), L21 (RPL21), L27a (RPL27A; 603637), and L28 (RPL28; 603638). Frigerio et al. (1995) completed the cDNA sequences of these human ribosomal proteins. The deduced 160-amino acid human RPL21 differs from rat Rpl21 by 3 amino acids. Northern blot analysis suggested variable expression of RPL21 in colorectal cancers compared to adjacent normal tissues, although no correlation between the level of expression and the severity of the disease was found.MAPPING
By somatic cell hybrid and radiation hybrid mapping analyses, Kenmochi et al. (1998) mapped the human RPL21 gene to chromosome 13q.MOLECULAR GENETICS
In affected members of 2 unrelated 4-generation Chinese families segregating autosomal dominant hypotrichosis simplex mapping to chromosome 13q12 (HYPT12; 615885), Zhou et al. (2011) identified heterozygosity for a missense mutation in the RPL21 gene (R32Q; 603636.0001).HISTORY
By use of somatic cell hybrids, Nakamichi et al. (1986) mapped a ribosomal protein gene to chromosome 8. They were not certain whether the gene was actually the RPL21 gene or a processed pseudogene. ... More on the omim web site
Nov. 16, 2018: Protein entry updated
Automatic update: OMIM entry 603636 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).