Protein transport protein Sec61 subunit gamma (SEC61G)
The protein contains 68 amino acids for an estimated molecular weight of 7741 Da.
Component of SEC61 channel-forming translocon complex that mediates transport of signal peptide-containing precursor polypeptides across endoplasmic reticulum (ER) (By similarity). Component of a ribosome-associated ER translocon complex involved in multi-pass membrane protein transport into the ER membrane and biogenesis (PubMed:32820719). The SEC61 channel cooperates with the translocating protein TRAM1 to import nascent proteins into the ER (By similarity). (updated: June 2, 2021)
Protein identification was indicated in the following studies:
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is predicted to be membranous by TOPCONS.
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No binding partner found
Biological Process
Posttranslational protein targeting to membrane, translocation
Protein insertion into ER membrane
Protein targeting to ER
The reference OMIM entry for this protein is 609215
Sec61 complex, gamma subunit; sec61g
DESCRIPTION
SEC61G is a subunit of the heteromeric SEC61 complex, which also contains alpha (SEC61A1; 609213) and beta (SEC61B; 609214) subunits. The SEC61 complex forms the core of the mammalian endoplasmic reticulum (ER) translocon, a transmembrane channel for the translocation of proteins across the ER membrane (Greenfield and High, 1999).CLONING
Hartmann et al. (1994) purified the Sec61 complex from canine pancreatic microsomes. Using degenerate oligonucleotides based on the peptide sequences to probe a canine kidney cell cDNA library, they cloned canine Sec61g. The deduced 68-amino acid protein has a calculated molecular mass of about 7.5 kD. Zhang et al. (2000) obtained a human SEC61G cDNA during large-scale sequencing of clones obtained from human CD34 (142230)-positive cell lines. The deduced protein contains 68 amino acids. Microarray analysis detected SEC61G expression in all CD34-positive cell lines examined.GENE FUNCTION
By immunolocalization of fluorescence-tagged canine Sec61a transfected into COS-1 cells, Greenfield and High (1999) determined that the Sec61 complex distributed to both the ER and the ER-Golgi intermediate compartment, but not to the trans-Golgi network. Endogenous Sec61b and Sec61g showed the same distribution. Another translocon component, the glycoprotein Tram (see 605190) was also present in post-ER compartments, suggesting that the core components of the mammalian ER translocon are not permanently resident in the ER, but rather they are maintained in the ER by a specific retrieval mechanism.MAPPING
Hartz (2005) mapped the SEC61G gene to chromosome 7p11.2 based on an alignment of the SEC61G sequence (GenBank GENBANK AF054184) with the genomic sequence. ... More on the omim web site
July 1, 2021: Protein entry updated
Automatic update: Entry updated from uniprot information.
April 10, 2021: Protein entry updated
Automatic update: Entry updated from uniprot information.
June 30, 2020: Protein entry updated
Automatic update: OMIM entry 609215 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).