60S ribosomal protein L15 (RPL15)
The protein contains 204 amino acids for an estimated molecular weight of 24146 Da.
No function (updated: Jan. 23, 2007)
Protein identification was indicated in the following studies:
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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Biological Process
Cytoplasmic translation
Nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
Response to ethanol
RRNA processing
SRP-dependent cotranslational protein targeting to membrane
Translation
Translational initiation
Viral transcription
Cellular Component
A band
Cytosol
Cytosolic large ribosomal subunit
Cytosolic ribosome
Membrane
Nucleus
Ribosome
The reference OMIM entry for this protein is 604174
Ribosomal protein l15; rpl15
DESCRIPTION
The mammalian ribosome is composed of 4 RNA species (see 180450) and approximately 80 different proteins (see 180466).CLONING
Adams et al. (1992) isolated an RPL15 cDNA as a human brain EST that shows sequence similarity to the yeast ribosomal protein YL10 gene. The complete coding sequence of the human RPL15 gene has been deposited in GenBank (GENBANK L25899). The deduced RPL15 protein has 205 amino acids.MAPPING
By somatic cell hybrid and radiation hybrid mapping analyses, Kenmochi et al. (1998) mapped the human RPL15 gene to 3p. Gross (2013) mapped the RPL15 gene to chromosome 3p24.2 based on an alignment of the RPL15 sequence (GenBank GENBANK AF279903) with the genomic sequence (GRCh37).MOLECULAR GENETICS
Landowski et al. (2013) performed array CGH for copy number variation in 87 probands with Diamond-Blackfan anemia (DBA12; 615550) who were negative for mutation in 10 known DBA-associated ribosomal protein genes, and identified a large deletion in the RPL15 gene in 1 patient (604174.0001). ... More on the omim web site
Nov. 16, 2018: Protein entry updated
Automatic update: OMIM entry 604174 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).