The reference OMIM entry for this protein is 117143

Centromeric protein e; cenpe
Kinesin family member 10; kif10

DESCRIPTION

The CENPE gene encodes a large kinetochore-associated kinesin-like motor protein required for spindle microtubule capture and attachment at the kinetochore during cell division (summary by Mirzaa et al., 2014).

CLONING

Yen et al. (1991) identified a 250- to 300-kD human centromere-associated protein, CENPE, by preparing monoclonal antibodies against a fraction of HeLa chromosome scaffold proteins enriched for centromere/kinetochore components. In cells progressing through different parts of the cell cycle, the localization of CENPE differs markedly from that observed for the previously identified centromere proteins CENPA (117139), CENPB (117140), CENPC (117141), and CENPD (117142). In contrast to these antigens, no monoclonal antibody staining was detected during interphase, and staining first appeared at the centromere region of chromosomes during prometaphase. Microinjection of the monoclonal antibody 177, which demonstrated CENPE, into metaphase cells blocked or significantly delayed progression into anaphase, although the morphology of the spindle and the configuration of the metaphase chromosomes appeared normal in these metaphase-arrested cells. Thus, CENPE function is required for the transition from metaphase to anaphase.

MAPPING

Testa et al. (1994) used CENPE cDNA to map the human gene to chromosome 4q24-q25 by fluorescence in situ hybridization. Gross (2014) mapped the CENPE gene to chromosome 4q24 based on an alignment of the CENPE sequence (GenBank GENBANK AB209996) with the genomic sequence (GRCh38).

NOMENCLATURE

Lawrence et al. (2004) presented a standardized kinesin nomenclature based on 14 family designations. Under this system, CENPE, or KIF10, belongs to the kinesin-7 family.

GENE FUNCTION

Yen et al. (1992) identified CENPE as a kinesin-like motor protein (Mr 312,000) that accumulates in the G2 phase of the cell cycle. CENPE associates with kinetochores during congression, relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division. CENPE is probably one of the motors responsible for mammalian chromosome movement and/or spindle elongation. Wood et al. (1997) cloned the Xenopus CENPE, which shows 74% identity with the human homolog. This protein associates with Xenopus centromeres in vivo and in vitro, and is required for metaphase chromosome alignment. Addition of anti-Xenopus CENPE antibodies disrupts metaphase chromosome alignment. Wood et al. (1997) further demonstrated that Xenopus CENPE powers chromosome movement towards microtubule plus ends in vitro. These data support a model in which CENPE functions in congression to tether kinetochores to microtubule plus ends. Yao et al. (2000) investigated the function of CENPE in attachment of kinetochores to spindle microtubules, alignment of chromosomes, and checkpoint signaling, using antisense oligonucleotides to suppress its synthesis and accumulation. They showed that CENPE is essential for stable, bioriented attachment of chromosomes to spindle microtubules, for development of tension across aligned chromosomes, for stabilization of spindle poles, and for satisfying the mitotic checkpoint. Using Xenopus egg extracts, Abrieu et al. (2000) found that CENPE is required for establishing and maintaining a checkpoint that delays anaphase onset until all centromeres are correctly attached to the mitotic spindle. When CENPE function was disrupted by immunode ... More on the omim web site

Subscribe to this protein entry history

Nov. 17, 2018: Protein entry updated
Automatic update: OMIM entry 117143 was added.

Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).