Keratin, type I cuticular Ha3-II (KRT33B)
The protein contains 404 amino acids for an estimated molecular weight of 46214 Da.
No function (updated: Sept. 12, 2018)
Protein identification was indicated in the following studies:
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
(right-click above to access to more options from the contextual menu)
No binding partner found
The reference OMIM entry for this protein is 602762
Keratin 33b, type i; krt33b
K33b
Ka28
Keratin, hair, acidic, 3b; krtha3b
Keratin, hard, type i, 3ii; ha3ii
DESCRIPTION
See KRTHA1 (601077) for general information on hair keratins.CLONING
By screening a human scalp cDNA library with a mouse Ha2 (see 602760) cDNA, Rogers et al. (1994) cloned a partial cDNA encoding KRTHA3B, which they named HA3II. The cDNA has an open reading frame that encodes 362 amino acids, including a predicted 37-amino acid C-terminal domain; the authors estimated that the cDNA lacks the coding sequence for 43 N-terminal residues. The partial HA3II isoform differs from the HA3I isoform (KRTHA3A; 602761) by 24 amino acids, 8 of which are in the center of the C-terminal domain. Using PCR, sequence, and Southern blot analyses, Rogers et al. (1994) demonstrated that HA3I and HA3II are distinct, single-copy genes. Rogers et al. (1998) reported that the deduced KRTHA3B protein has 405 amino acids and shares 93.3% amino acid sequence identity with KRTHA3A. By RT-PCR, Rogers et al. (1998) showed that KRTHA3B is expressed in the human hair follicle. See Langbein et al. (1999) for further details on the expression pattern of the KRTHA3B gene in the hair follicle.GENE STRUCTURE
Rogers et al. (1998) reported that the KRTHA3B gene contains 7 exons.MAPPING
Rogers et al. (1998) isolated and characterized 2 overlapping human PAC clones that cover 190 kb on 17q12-q21 and contain 9 type I hair keratin genes, 1 transcribed hair keratin pseudogene, and 1 orphan exon. The order of the genes is 5-prime--KRTHA6 (604540)--KRTHA5 (602764)--KRTHA2 (602760)--orphan exon--KRTHA8 (604542)--KRTHA7 (604541)--pseudogene--KRTHA1--KRTHA4 (602763)--KRTHA3B--KRTHA3A--3-prime. The hair keratin genes range in size from 4.2 to 7.5 kb, and the genes are separated from each other by 5.5 to 18.4 kb; all are located within about 140 kb. Each gene is transcribed from the 5-prime to 3-prime direction. Based on sequence homologies, the genes can be grouped into 3 subclusters of tandemly arranged genes. One subcluster, group A, consists of KRTHA1, KRTHA3A, KRTHA3B, and KRTHA4, which share 89% overall amino acid identity. A second subcluster, group B, contains KRTHA7 and KRTHA8, as well as the hair keratin pseudogene, which the authors called HAA. The functional hair keratins and hypothetical HAA hair keratin share approximately 81% overall amino acid identity. The third subcluster, group C, consists of the structurally less related hair keratins KRTHA2, KRTHA5, and KRTHA6, which share about 70% amino acid identity. ... More on the omim web site
Oct. 20, 2018: Protein entry updated
Automatic update: OMIM entry 602762 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).