Fibrous sheath CABYR-binding protein (FSCB)
The protein contains 825 amino acids for an estimated molecular weight of 87956 Da.
May be involved in the later stages of fibrous sheath biogenesis and spermatozoa capacitation. Inhibits ROPN1 and ROPN1L SUMOylation. Binds calcium. (updated: Sept. 12, 2018)
Protein identification was indicated in the following studies:
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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No binding partner found
The reference OMIM entry for this protein is 611779
Fibrous sheath cabyr-binding protein
C14orf155
CLONING
Using coimmunoprecipitation experiments of mouse sperm protein lysates with an antibody to CABYR (612135), Li et al. (2007) identified a novel peptide, which they designated Fscb (fibrous sheath CABYR-binding protein). Antibodies were raised to a truncated version of the mouse protein, whose full-length version was predicted to be 1,074 amino acids. By database analysis, Li et al. (2007) identified human FSCB, which encodes a deduced 825-amino acid protein. Northern blot analysis of mouse tissues detected a 3.5-kb testis-specific Fscb transcript.GENE FUNCTION
Li et al. (2007) showed that Fscb is first expressed in the elongating spermatids during spermatogenesis. Later in spermatogenesis it is found in the flagellum where it was shown to localize to a cortical layer at the surface of the ribs and longitudinal columns of the fibrous sheath. Li et al. (2007) postulated that FSCB may be involved in later stages of fibrous sheath assembly. Like CABYR, FSCB is a calcium-binding protein, and both recombinant and native FSCB are phosphorylated by protein kinase A (PKA; see 176911).MAPPING
By sequence analysis, Li et al. (2007) mapped the FSCB gene to chromosome 14q21.3 and the mouse homolog to chromosome 12. ... More on the omim web site
Oct. 19, 2018: Protein entry updated
Automatic update: OMIM entry 611779 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).