SCY1-like protein 2 (SCYL2)
The protein contains 929 amino acids for an estimated molecular weight of 103709 Da.
Component of the AP2-containing clathrin coat that may regulate clathrin-dependent trafficking at plasma membrane, TGN and endosomal system (Probable). A possible serine/threonine-protein kinase toward the beta2-subunit of the plasma membrane adapter complex AP2 and other proteins in presence of poly-L-lysine has not been confirmed (PubMed:15809293, PubMed:16914521). By regulating the expression of excitatory receptors at synapses, plays an essential role in neuronal function and signaling and in brain development (By similarity). (updated: Feb. 10, 2021)
Protein identification was indicated in the following studies:
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs33968174 | |
| dbSNP:rs763873645 | |
| a lung adenocarcinoma sample; somatic mutation |
Biological Process
Brain development
Endosome to lysosome transport
Negative regulation of canonical Wnt signaling pathway
Positive regulation of clathrin-dependent endocytosis
Positive regulation of receptor internalization
Pyramidal neuron development
Receptor internalization involved in canonical Wnt signaling pathway
Cellular Component
Clathrin-coated vesicle
Endosome membrane
Golgi apparatus
Perinuclear region of cytoplasm
The reference OMIM entry for this protein is 616365
Scy1-like protein 2; scyl2
Scy1, s. cerevisiae, homolog of, 2
Coated vesicle-associated kinase, 104-kd; cvak104
Kiaa1360
DESCRIPTION
SCYL2 is a serine/threonine kinase that is expressed in clathrin-coated vesicles (Conner and Schmid, 2005).CLONING
By searching for cDNAs encoding large proteins expressed in brain, Nagase et al. (2000) obtained a partial cDNA encoding SCYL2, which they called KIAA1360. The deduced 796-amino acid sequence contains a kinase domain. RT-PCR detected high expression in lung and moderate to high expression in all other adult and fetal tissues and specific brain regions tested. By directed proteomic and mass spectrometric analysis of adaptor protein preparations from bovine brain, followed by EST database analysis, Conner and Schmid (2005) obtained a full-length cDNA encoding human SCYL2, which they called CVAK104. The deduced 933-amino acid protein has a calculated molecular mass of 104 kD. It contains an N-terminal serine/threonine kinase domain, followed by 2 weak clathrin-binding motifs (DLL), a predicted coiled-coil domain, and an NPF motif that mediates interactions with EPS15 (600015) homology domains. Human CVAK104 shares about 93% amino acid identity with its mouse and rat homologs. Immunoblot analysis of bovine brain lysates detected a 104-kD protein that was predominantly membrane associated and cofractionated with isolated clathrin-coated vesicles. Two other proteins of 116 and 118 kD were present in both cytosol and membrane fractions, but neither associated with clathrin-coated vesicles.GENE FUNCTION
Conner and Schmid (2005) found that human CVAK104 bound directly to both clathrin and the plasma membrane adaptor AP2. Kinase assays showed that CVAK104 phosphorylated itself and the beta-2 adaptin subunit (AP2B1; 601025) of AP2 in a poly(L)-lysine-stimulated manner.MAPPING
Gross (2015) mapped the SCYL2 to chromosome 12q23.1 based on an alignment of the SCYL2 sequence (GenBank GENBANK BC063798) with the genomic sequence (GRCh38). ... More on the omim web site
Feb. 16, 2021: Protein entry updated
Automatic update: Entry updated from uniprot information.
Oct. 20, 2018: Protein entry updated
Automatic update: OMIM entry 616365 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).