POTE ankyrin domain family member E (POTEE)
The protein contains 1075 amino acids for an estimated molecular weight of 121363 Da.
No function (updated: Oct. 10, 2018)
Protein identification was indicated in the following studies:
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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No binding partner found
Molecular Function
The reference OMIM entry for this protein is 608914
Pote ankyrin domain family, member e; potee
Prostate-, ovary-, testis-, and placenta-expressed gene on chromosome 1; pote2
Pote2-gamma
CLONING
Bera et al. (2002) identified 10 paralogs of the POTE gene (607549), including POTE2. By partially sequencing clones obtained from prostate and testis cDNA libraries, they determined that the POTE2 gene is transcribed. By PCR, Bera et al. (2004) cloned 4 full-length splice variants of POTE2 from normal prostate cDNA. The longest deduced protein, POTE2A, contains 712 amino acids and has a calculated molecular mass of 72.5 kD. POTE2A has 3 cysteine-rich N-terminal repeats, followed by 7 ankyrin repeats and a C-terminal helical region. The deduced 394-amino acid POTE2B protein has a calculated molecular mass of 40.2 kD. Compared with POTE2A, POTE2B has extra sequence within the ankyrin repeat region and lacks the C-terminal helical region. POTE2C and POTE2D differ only in the long 3-prime UTR. The deduced 376-amino acid POTE2C/POTE2D protein has a calculated molecular mass of 38.6 kD. Compared with POTE2A, POTE2C/POTE2D lacks the C-terminal helical region. In vitro transcription and translation of the POTE2 variants resulted in proteins with molecular masses in agreement with their calculated masses. By genomic sequence analysis, Hahn et al. (2006) identified 5 POTE2 genes, which they called POTE2-alpha, -beta, -gamma, -delta, and -beta-prime, on chromosome 2. POTE2-beta and -beta-prime are identical. The POTE2 splice variants reported by Bera et al. (2004) are encoded by the POTE2-gamma gene. Based on sequence similarity, the POTE genes can be divided into 3 groups. Lee et al. (2006) determined that members of group 3, which includes the POTE2 genes, have an insertion of a beta-actin (102630) retrogene at their 3-prime ends, just prior to a LINE element that is present in all POTE genes. The actin sequence is included within the ORFs of POTE2-alpha, POTE2-beta, and POTE2-gamma. In the other members of group 3, a stop codon before the actin module results in premature termination.GENE STRUCTURE
Bera et al. (2004) determined that the POTE2 gene has 15 exons and spans about 45 kb.MAPPING
By genomic sequence analysis, Bera et al. (2002) mapped paralogs of the POTE gene to chromosomes 2q22.2 and 2q14.3. Using FISH, they mapped POTE2 to chromosome 2q13-q22. By genomic sequence analysis, Hahn et al. (2006) identified 5 POTE2 genes within a 2-Mb region of chromosome 2q21.1. Two of the genes, POTE2-beta and -beta-prime, are identical and are embedded in a long palindromic segment.EVOLUTION
Lee et al. (2006) reported that the POTE genes are found only in primates. They determined that the POTE genes entered the primate genome before the divergence of New World monkeys and Old World monkeys. A beta-actin sequence was inserted at the 3-prime end of an ancestral POTE gene before the divergence of Old World monkeys and apes and is now present in several POTE paralogs, including the POTE2 genes. ... More on the omim web site
Oct. 20, 2018: Protein entry updated
Automatic update: OMIM entry 608914 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).