Integral membrane protein GPR180 (GPR180)
The protein contains 440 amino acids for an estimated molecular weight of 49395 Da.
No function (updated: Sept. 12, 2018)
Protein identification was indicated in the following studies:
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is predicted to be membranous by TOPCONS.
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| Variant | Description |
|---|---|
| a breast cancer sample; somatic mutation |
Molecular Function
The reference OMIM entry for this protein is 607787
G protein-coupled receptor 180; gpr180
Intimal thickness-related receptor; itr
CLONING
By differential display, Tsukada et al. (2003) cloned rabbit Itr, which was upregulated early after aorta injury by balloon catheterization. They obtained a full-length human ITR cDNA by screening a heart cDNA library. The deduced 440-amino acid protein, which is produced predominantly in vascular smooth muscle cells, contains an N-terminal signal sequence, 7 transmembrane regions, and a signature motif found in members of the rhodopsin (180380)-like G protein-coupled receptor superfamily. The 3-prime untranslated region contains 3 putative polyadenylation sites. Northern blot analysis detected major transcripts of 1.9 and 3.8 kb that were differentially expressed in most tissues examined.GENE STRUCTURE
Iida et al. (2003) determined the genomic structure of the human ITR gene, which contains 9 exons spanning approximately 27 kb of genomic DNA. The first methionine codon of the open reading frame is located in exon 1; the termination codon and poly-A signal are in the last exon.MAPPING
By FISH, Tsukada et al. (2003) mapped the ITR gene to chromosome 13q31.MOLECULAR GENETICS
Iida et al. (2003) constructed a fine-scale map of 22 SNPs that were detected at the ITR locus in a 96-chromosome sample of the Japanese population. Only 2 of the 22 SNPs were in the coding region of the ITR gene. - Associations Pending Confirmation For discussion of a possible association between variation in the GPR180 gene and microcoria with goniodysgenesis, see 156600.ANIMAL MODEL
Tsukada et al. (2003) developed Itr-null mice. Mutant mice were indistinguishable from wildtype mice in appearance, growth rate, reproduction, and histology of major organs. However, following cuff placement around the femoral artery, Itr-null mice showed little of the neointima that formed in wildtype mice within the experimental period. The neointimal area was about 200% greater in wildtype mice than in Itr-null mice 14 days after cuff placement, and DNA synthesis in vascular smooth muscle cells was significantly suppressed in Itr-null mice. Because Itr-deficient mice were found to be resistant to experimental thickening of the intima, Tsukada et al. (2003) concluded that the product of this gene may play an important role in the regulation of vascular remodeling. ... More on the omim web site
June 30, 2020: Protein entry updated
Automatic update: OMIM entry 607787 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).