5'(3')-deoxyribonucleotidase, cytosolic type (NT5C)
The protein contains 201 amino acids for an estimated molecular weight of 23383 Da.
Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides, with a preference for dUMP and dTMP, intermediate activity towards dGMP, and low activity towards dCMP and dAMP. (updated: Oct. 10, 2018)
Protein identification was indicated in the following studies:
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs11541956 |
The reference OMIM entry for this protein is 191720
5-prime,3-prime-@nucleotidase, cytosolic; nt5c
Pyrimidine 5-prime nucleotidase 2; p5n2
Uridine 5-prime monophosphate hydrolase 2; umph2
Deoxyribonucleotidase, cytosolic, 1; dnt1
DESCRIPTION
Pyrimidine 5-prime nucleotidase (P5N; EC 3.1.3.5), also called uridine 5-prime monophosphate hydrolase (UMPH), catalyzes the dephosphorylation of the pyrimidine 5-prime monophosphates UMP and CMP to the corresponding nucleosides. There are 2 isozymes of pyrimidine 5-prime nucleotidase in red blood cells, referred to as type I (UMPH1; 606224) and type II (UMPH2).CLONING
Electrophoretic analysis shows a single band of UMPH enzyme activity in human cells but 2 distinct UMPH activities in rodent cells. Swallow et al. (1983) and Paglia et al. (1984) showed that in fact humans also possess 2 distinct UMPH isozymes that are not electrophoretically separable. This result was based on observations in persons who lacked UMPH1 but retained normal UMPH2 activity. Differences in substrate specificity distinguish the 2 isozymes. Deficiency of UMPH1 causes hemolytic anemia; see 266120. 5-prime(3-prime)-Deoxyribonucleotidase (DNT1) is a ubiquitous enzyme in mammalian cells with particularly high activity in lymphoid cells. Biochemically purified DNT1 has an apparent molecular mass of 45 kD and hydrolyzes 5-prime deoxyribonucleotides (dNTP) and 2-prime(3-prime)-dNTP and ribonucleotides but not 5-prime ribonucleotides (Hoglund and Reichard, 1990; Tjernshaugen and Fritzson, 1976). By micropeptide sequencing and database searching, Rampazzo et al. (2000) identified a full-length cDNA encoding mouse Dnt1. They also cloned a partial human DNT1 cDNA that lacked the 5-prime 181 bp present in the mouse sequence. The amino acid sequence predicted from the partial human cDNA is 83% identical to that of the mouse protein. Northern blot analysis revealed ubiquitous expression of 1.5- and 1.0-kb DNT1 transcripts, with highest expression in skeletal muscle, heart, and pancreas. SDS-PAGE and enzyme analysis showed that recombinant murine Dnt1 is expressed as a 25-kD enzyme that is active only when expressed from the first ATG and catalyzes nucleotide dephosphorylation, but lacks phosphotransferase activity. Recombinant human DNT1 was active only when fused with the 5-prime end of the mouse cDNA. Fluorescence microscopy indicated that DNT is expressed in the cytoplasm and nucleus and is not targeted to any specific organelle.MAPPING
Wilson et al. (1986) demonstrated in somatic cell hybrids that UMPH2 cosegregated with 17q. Using mouse-Syrian hamster hybrids, Wilson et al. (1987) demonstrated synteny of Umph2 and Glk in the mouse, indicating that the Umph2 gene is on mouse chromosome 11. By means of chromosome-mediated gene transfer, Xu et al. (1988) demonstrated that UMPH2 is in a group of genes that are cotransfected along with the selectable marker TK1 (188300), which lies in the region 17q23.2-q25.3. UMPH2 cotransfected with the GHC genes (see 139250) with a high frequency. The order was thought to be: cen--GAA--UMPH2--GHC--TK1--GALK--qter. By sequence analysis, Rampazzo et al. (2000) identified the DNT1 and DNT2 (605292) genes within genomic clones located on chromosome 17. They also found that the 2 genes have a similar genomic structure. ... More on the omim web site
May 12, 2019: Protein entry updated
Automatic update: OMIM entry 191720 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).