DnaJ homolog subfamily C member 9 (DNAJC9)
The protein contains 260 amino acids for an estimated molecular weight of 29910 Da.
May play a role as co-chaperone of the Hsp70 family proteins HSPA1A, HSPA1B and HSPA8. (updated: Sept. 12, 2018)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology, is annotated as membranous in UniProt.
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The reference OMIM entry for this protein is 611206
Dnaj/hsp40 homolog, subfamily c, member 9; dnajc9
J domain of dnaj-like protein 1; jdd1
Kiaa0974
CLONING
By sequencing clones obtained from a size-fractionated brain cDNA library, Nagase et al. (1999) cloned DNAJC9, which they designated KIAA0974. The transcript contains repetitive elements in its 3-prime UTR, and the deduced protein contains 565 amino acids. RT-PCR ELISA detected moderate expression in all tissues and specific brain regions examined, with highest levels in kidney, testis, ovary, and fetal liver. Takamura et al. (2001) cloned rat Dnajc9, which they called Jdd1. In situ hybridization revealed strong Jdd1 expression in the germinal zone of rat central nervous system, and Jdd1 expression continued after birth. Jdd1 expression was also detected in embryonic rat liver, lung, and kidney cortex.MAPPING
The International Radiation Hybrid Mapping Consortium mapped the DNAJC9 gene to chromosome 10 (TMAP SHGC-35170). ... More on the omim web site
Feb. 23, 2019: Protein entry updated
Automatic update: model status changed
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).
Oct. 19, 2018: Protein entry updated
Automatic update: OMIM entry 611206 was added.