Protein YIPF5 (YIPF5)
The protein contains 257 amino acids for an estimated molecular weight of 27989 Da.
Plays a role in transport between endoplasmic reticulum and Golgi. (updated: Sept. 12, 2018)
Protein identification was indicated in the following studies:
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is predicted to be membranous by TOPCONS.
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Biological Process
Protein transport
Regulation of ER to Golgi vesicle-mediated transport
Vesicle-mediated transport
Cellular Component
COPII-coated ER to Golgi transport vesicle
Endoplasmic reticulum
Endoplasmic reticulum exit site
Endoplasmic reticulum membrane
Golgi apparatus
Integral component of membrane
Intracellular membrane-bounded organelle
Nucleoplasm
Molecular Function
The reference OMIM entry for this protein is 611483
Yip1 domain family, member 5; yipf5
Smooth muscle cell-associated protein 5; smap5
Yip1a
CLONING
By RT-PCR and 3-prime RACE of a human coronary smooth muscle cell RNA, Stolle et al. (2005) cloned YIPF5, which they called SMAP5. By searching EST databases and by exon-specific PCR, they identified 9 splice variants. The most common transcript encodes a deduced 257- amino acid protein with 4 or 5 transmembrane domains forming a characteristic Rab GTPase-interacting factor domain. Northern blot analysis detected a major 2-kb transcript expressed at highest levels in liver, heart, skeletal muscle, kidney, and small intestine with low to no expression in other tissues analyzed. Heart and skeletal muscle expressed an additional 7-kb transcript. RT-PCR analysis detected highest expression in coronary smooth muscle cells, followed by duodenum, heart, ileum, jejunum, pancreas, prostate, retina, small intestine, uterus, fibroblasts, and colon and lung tumor. SMAP5 colocalized with a cis-Golgi marker in transfected HeLa cells. By PCR of a human testis cDNA library, Jin et al. (2005) independently cloned YIPF5, which they called YIP1A.GENE FUNCTION
Stolle et al. (2005) showed that YIPF5 was significantly induced by TGFB1 (190180) in coronary smooth muscle cells but not in fibroblasts. Using reciprocal yeast 2-hybrid analysis and coimmunoprecipitation assays, Jin et al. (2005) showed that YIP1A and YIF1A (611484) interact directly. Both proteins localized to the Golgi apparatus in transfected human embryonic kidney cells, and the Golgi localization of YIF1A depended upon the transmembrane domain of YIP1A.GENE STRUCTURE
Stolle et al. (2005) determined that the YIPF5 gene contains 6 coding exons and spans about 12.5 kb. The promoter region is GC-rich with 10 putative SP1 (189906)-binding sites but lacks a TATA box.MAPPING
By genomic sequence analysis, Stolle et al. (2005) mapped the YIPF5 gene to chromosome 5q32. ... More on the omim web site
June 30, 2020: Protein entry updated
Automatic update: OMIM entry 611483 was added.
Dec. 10, 2018: Protein entry updated
Automatic update: model status changed
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).