GPI transamidase component PIG-S (PIGS)
The protein contains 555 amino acids for an estimated molecular weight of 61656 Da.
Component of the GPI transamidase complex. Essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates. (updated: Oct. 10, 2018)
Protein identification was indicated in the following studies:
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is predicted to be membranous by TOPCONS.
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| Variant | Description |
|---|---|
| a breast cancer sample; somatic mutation | |
| dbSNP:rs34669811 | |
| GPIBD18 | |
| GPIBD18 |
The reference OMIM entry for this protein is 610271
Phosphatidylinositol glycan, class s; pigs
DESCRIPTION
Glycosylphosphatidylinositol (GPI) is a membrane anchor for cell surface proteins. PIGS is a subunit of the GPI transamidase complex that catalyzes the attachment of preformed GPI to proteins containing a C-terminal GPI attachment signal (Ohishi et al., 2001).CLONING
Ohishi et al. (2001) purified PIGS from the GPI transamidase complex isolated from a human myelogenous leukemia cell line. The full-length PIGS cDNA encodes a deduced 555-amino acid protein with 2 transmembrane domains near the N and C termini.GENE FUNCTION
By disruption of the Pigs gene in a mouse embryonal carcinoma cell line, Ohishi et al. (2001) found that Pigs is not required for GPI synthesis but is essential for attachment of GPI to proteins. During the GPI attachment process, GPI transamidase forms a carbonyl intermediate with the precursor protein, and in the absence of Pigs, the carbonyl intermediate was not generated. The lack of Pigs did not affect the expression of the other GPI transaminase subunits Gaa1 (GPAA1; 603048), Gpi8 (PIGK; 605087), or Pigt (610272). Coimmunoprecipitation experiments indicated that PIGS and PIGT associate with each other.MAPPING
The International Radiation Hybrid Mapping Consortium mapped the PIGS gene to chromosome 17 (TMAP RH17002). ... More on the omim web site
June 30, 2020: Protein entry updated
Automatic update: OMIM entry 610271 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).