Protein canopy homolog 3 (CNPY3)
The protein contains 278 amino acids for an estimated molecular weight of 30748 Da.
Toll-like receptor (TLR)-specific co-chaperone for HSP90B1. Required for proper TLR folding, except that of TLR3, and hence controls TLR exit from the endoplasmic reticulum. Consequently, required for both innate and adaptive immune responses (By similarity). (updated: June 1, 2001)
Protein identification was indicated in the following studies:
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| DEE60 | |
| dbSNP:rs1063252 | |
| dbSNP:rs9471969 |
The reference OMIM entry for this protein is 610774
Canopy 3, zebrafish, homolog of; cnpy3
Trinucleotide repeat-containing gene 5; tnrc5
Protein associated with toll-like receptor 4, a; prat4a
Protein associated with tlr4, a
Expanded repeat domain, cag/ctg, 5; erda5
DESCRIPTION
PRAT4A is associated with the immature form of TLR4 (603030) and regulates its cell surface expression (Wakabayashi et al., 2006).CLONING
By immunoprecipitation analysis of an embryonic kidney cell line, Wakabayashi et al. (2006) identified TNRC5, which they called PRAT4A, as a 40-kD protein that coprecipitated with TLR4 and MD2 (605243). The 278-amino acid PRAT4A protein has a 37-amino acid N-terminal signal sequence, a canonical N-glycosylation site, and 6 cys residues. It shares significant homology with mouse Prat4a and human PRAT4B (610047). Northern blot analysis detected Prat4a expression in all mouse tissues examined.GENE FUNCTION
Using RNA interference in human and mouse cell lines, Wakabayashi et al. (2006) demonstrated that knockdown of PRAT4A diminished TLR4/MD2 expression, as well as lipopolysaccharide responsiveness. Human PRAT4A bound hypoglycosylated mouse Tlr4 with or without Md2, but it did not bind mature Tlr4/Md2, in human and mouse cells. Wakabayashi et al. (2006) proposed that PRAT4A has a role in TLR4 maturation and glycosylation.MAPPING
The International Radiation Hybrid Mapping Consortium mapped the CNPY3 gene to chromosome 6 (TMAP RH18283). ... More on the omim web site
Oct. 20, 2018: Protein entry updated
Automatic update: OMIM entry 610774 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).