Rab GTPase-binding effector protein 2 (RABEP2)
The protein contains 569 amino acids for an estimated molecular weight of 63543 Da.
Plays a role in membrane trafficking and in homotypic early endosome fusion (PubMed:9524116). Participates in arteriogenesis by regulating vascular endothelial growth factor receptor 2/VEGFR2 cell surface expression and endosomal trafficking (PubMed:29425100). By interacting with SDCCAG8, localizes to centrosomes and plays a critical role in ciliogenesis (PubMed:27224062). (updated: May 8, 2019)
Protein identification was indicated in the following studies:
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
(right-click above to access to more options from the contextual menu)
Biological Process
Cell projection organization
Endocytosis
Positive regulation of GTPase activity
Protein transport
Regulation of cilium assembly
Cellular Component
Centrosome
Ciliary basal body
Cytosol
Early endosome
Intracellular membrane-bounded organelle
The reference OMIM entry for this protein is 611869
Rabaptin, rab gtpase-binding effector protein 2; rabep2
Rabaptin 5-beta
CLONING
Using a yeast 2-hybrid system, Gournier et al. (1998) identified a novel rat gene, which they called rabaptin-5-beta, encoding a 62-kD protein that interacts with the GTP-bound form of RAB5 (179512), a small GTPase involved in signal transduction and mitogenesis. The deduced protein, RABEP2, shares 42% sequence identity with rat RABEP1 (603616). Both proteins contain coiled-coil repeats and are recruited on the endosomal membrane by RAB5 in the presence of GTP.GENE FUNCTION
Gournier et al. (1998) found that RABEP2 does not form heterodimers with RABEP1 but does complex with RAB guanine nucleotide exchange factor-1 (RABGEF1; 609700). Immunodepletion with an antibody against the RABEP2 complex resulted in partial inhibition of early endosome fusion. Gournier et al. (1998) concluded that RAB5 binds to both RABEP1 and RABEP2 for endocytic membrane docking and fusion.MAPPING
Scott (2008) mapped the RABEP2 gene to chromosome 16p11.2 based on an alignment of the RABEP2 sequence (GenBank GENBANK AC109460) with the genomic sequence (build 36.2). ... More on the omim web site
May 11, 2019: Protein entry updated
Automatic update: Entry updated from uniprot information.
Dec. 10, 2018: Protein entry updated
Automatic update: model status changed
Oct. 20, 2018: Protein entry updated
Automatic update: OMIM entry 611869 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).