1-acyl-sn-glycerol-3-phosphate acyltransferase delta (AGPAT4)
The protein contains 378 amino acids for an estimated molecular weight of 44021 Da.
Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (By similarity). Exhibits high acyl-CoA specificity for polyunsaturated fatty acyl-CoA, especially docosahexaenoyl-CoA (22:6-CoA, DHA-CoA) (By similarity). (updated: July 3, 2019)
Protein identification was indicated in the following studies:
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is predicted to be membranous by TOPCONS.
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No binding partner found
Biological Process
CDP-diacylglycerol biosynthetic process
Phosphatidic acid biosynthetic process
Phospholipid biosynthetic process
Phospholipid metabolic process
The reference OMIM entry for this protein is 614795
1-@acylglycerol-3-phosphate o-acyltransferase 4; agpat4
1-@acyl-sn-glycerol 3-phosphate acyltransferase 4
Lysophosphatidic acid acyltransferase, delta
Lpaat-delta
DESCRIPTION
AGPAT4 is a member of a family of 1-acyl-sn-glycerol 3-phosphate acyltransferases (EC 2.3.1.51), also known as lysophosphatidic acid acyltransferases, that catalyze the acylation of lysophosphatidic acid to phosphatidic acid, the precursor of all glycerolipids (summary by Lu et al., 2005).CLONING
Dominguez et al. (1998) identified ED166-4A1, an AGPAT4 cDNA, among a collection of AU-rich element (ARE)-containing mRNAs. AREs are cis-acting sequences typically found in 3-prime untranslated regions of many labile mRNAs. AREs either mediate rapid degradation of mRNA or inhibit its translation. Lu et al. (2005) cloned mouse Agpat4. The deduced 378-amino acid protein has a putative N-terminal signal sequence and 3 transmembrane domains. It contains catalytic and substrate-binding motifs and a third motif conserved among AGPAT family members. RT-PCR analysis revealed high Agpat4 expression in mouse brain and intermediate to low expression in skeletal muscle, gut, kidney, spleen, and lung. Little to no Agpat4 was detected in heart and liver. Using real-time PCR, Prasad et al. (2011) found that AGPAT4 was variably expressed in all human tissues examined, with highest expression in skeletal muscle, followed by heart, liver, prostate, and thymus.MAPPING
Hartz (2012) mapped the AGPAT4 gene to chromosome 6q26 based on an alignment of the AGPAT4 sequence (GenBank GENBANK AF156776) with the genomic sequence (GRCh37). ... More on the omim web site
July 4, 2019: Protein entry updated
Automatic update: Entry updated from uniprot information.
June 7, 2019: Protein entry updated
Automatic update: OMIM entry 614795 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).