Cell division cycle protein 23 homolog (CDC23)
The protein contains 597 amino acids for an estimated molecular weight of 68834 Da.
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. (updated: Oct. 10, 2018)
Protein identification was indicated in the following studies:
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs2231471 | |
| dbSNP:rs17228304 |
Biological Process
Anaphase-promoting complex-dependent catabolic process
Cell division
Metaphase/anaphase transition of mitotic cell cycle
Mitotic cell cycle
Mitotic metaphase plate congression
Positive regulation of mitotic metaphase/anaphase transition
Protein K11-linked ubiquitination
Protein ubiquitination
Regulation of exit from mitosis
Regulation of mitotic cell cycle phase transition
Regulation of mitotic metaphase/anaphase transition
Ubiquitin-dependent protein catabolic process
The reference OMIM entry for this protein is 603462
Cell division cycle 23; cdc23
Anaphase-promoting complex, subunit 8; apc8
Anapc8
DESCRIPTION
CDC23 is a subunit of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle (summary by Jorgensen et al., 2001).CLONING
By searching EST databases with peptide sequences from Xenopus APC subunits, Yu et al. (1998) identified partial cDNAs encoding human APC2 (606946), APC4 (606947), APC5 (606948), APC7 (606949), and CDC23. Zhao et al. (1998) stated that CDC23, CDC27 (116946), APC7, and CDC16 (603461) are members of the TPR (tetratricopeptide repeat) family. The TPR is an imperfect 34-amino acid motif implicated in protein-protein interactions (see 601963). These authors reported that the predicted 591-amino acid human CDC23 protein shares 30% protein sequence identity with S. cerevisiae CDC23. Both the human and yeast proteins contain 9 TPR units, which are arranged as a single repeat followed by a block of 8 tandem repeats. Northern blot analysis revealed that the approximately 3.3-kb CDC23 mRNA is expressed ubiquitously.GENE STRUCTURE
The CDC23 gene contains 16 exons spanning 31 kb (Zhao et al., 1998).MAPPING
By genomic sequence analysis and fluorescence in situ hybridization, Zhao et al. (1998) mapped the CDC23 gene to chromosome 5q31.1. ... More on the omim web site
June 30, 2020: Protein entry updated
Automatic update: OMIM entry 603462 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).