Anaphase-promoting complex subunit 4 (ANAPC4)
The protein contains 808 amino acids for an estimated molecular weight of 92116 Da.
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. (updated: Oct. 10, 2018)
Protein identification was indicated in the following studies:
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| a colorectal cancer sample; somatic mutation | |
| dbSNP:rs34811474 | |
| dbSNP:rs11550697 |
No binding partner found
Biological Process
Anaphase-promoting complex-dependent catabolic process
Cell cycle
Cell division
Positive regulation of mitotic metaphase/anaphase transition
Protein K11-linked ubiquitination
Regulation of exit from mitosis
Regulation of mitotic cell cycle phase transition
Ubiquitin-dependent protein catabolic process
The reference OMIM entry for this protein is 606947
Anaphase-promoting complex, subunit 4; anapc4
Apc4
DESCRIPTION
ANAPC4 is a subunit of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle (summary by Jorgensen et al., 2001).CLONING
By searching an EST database using frog APC peptide sequences, Yu et al. (1998) obtained full-length cDNAs encoding APC2 (606946), APC4, APC5 (606948), and APC7 (606949). The deduced 809-amino acid APC4 protein did not appear to have a yeast homolog. Yu et al. (1998) stated that the APC is highly conserved from yeast to humans.MAPPING
The International Radiation Hybrid Mapping Consortium mapped the ANAPC4 gene to chromosome 4 (TMAP SHGC-8588). ... More on the omim web site
June 29, 2020: Protein entry updated
Automatic update: OMIM entry 606947 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).