Mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5)
The protein contains 846 amino acids for an estimated molecular weight of 95024 Da.
May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. (updated: Sept. 12, 2018)
Protein identification was indicated in the following studies:
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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The reference OMIM entry for this protein is 604923
Mitogen-activated protein kinase kinase kinase kinase 5; map4k5
Mapkkkk5
DESCRIPTION
MAP4K5 is a member of the SPS1/STE20 family of kinases, which is involved in relaying signals from G proteins to the cytosolic MAP kinases (Tung and Blenis, 1997).CLONING
Homologs of the S. cerevisiae STE20 and SPS1 proteins are predicted to link the membrane with the cytoplasmic signaling machinery; see 602255. By screening a T-cell library with a PCR-derived probe that was similar to yeast STE20, Tung and Blenis (1997) obtained a cDNA encoding MAP4K5, which they called 'kinase homologous to SPS1/STE20,' or KHS. Sequence analysis revealed that the 846-amino acid MAP4K5 protein contains an N-terminal catalytic domain and shares 55% amino acid identity with MAP4K2 (603166). Northern blot analysis detected a 4.5-kb MAP4K5 transcript in all tissues tested, namely spleen, thymus, prostate, testis, ovary, small intestine, colon, and peripheral blood leukocytes. SDS-PAGE and immunoblot analyses determined that MAP4K5 is expressed as a 95-kD protein. Confocal laser microscopy demonstrated cytoplasmic expression of MAP4K5. Functional analyses showed that MAP4K5 has kinase activity and that it activates JNK (MAPK8; 601158) but not ERK1 (MAPK3; 601795) or MAPK14 (600289) through MAP2K4.MAPPING
The International Radiation Hybrid Mapping Consortium mapped the MAP4K5 gene to chromosome 14 (TMAP stSG36044). ... More on the omim web site
June 30, 2020: Protein entry updated
Automatic update: OMIM entry 604923 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).