Dolichyl-phosphate beta-glucosyltransferase (ALG5)
The protein contains 324 amino acids for an estimated molecular weight of 36946 Da.
No function (updated: Sept. 12, 2018)
Protein identification was indicated in the following studies:
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is predicted to be membranous by TOPCONS.
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No binding partner found
The reference OMIM entry for this protein is 604565
Alg5, s. cerevisiae, homolog of; alg5
Dolichyl phosphate glucosyltransferase
DESCRIPTION
The ALG5 gene encodes dolichyl phosphate glucosyltransferase (EC 2.4.1.117).CLONING
By searching EST databases and using low-stringency PCR amplification to screen a human T-lymphocyte cDNA library, Imbach et al. (1999) obtained a complete cDNA encoding the ortholog of S. cerevisiae ALG5. The human ALG5 protein contains 242 amino acids and is 58% similar to yeast ALG5. Northern blot analysis revealed expression of a 1.3-kb ALG5 transcript in pancreas, placenta, liver, heart, brain, kidney, skeletal muscle, and lung.MAPPING
Gross (2014) mapped the ALG5 gene to chromosome 13q13.3 based on an alignment of the ALG5 sequence (GenBank GENBANK AF161498) with the genomic sequence (GRCh37). ... More on the omim web site
June 30, 2020: Protein entry updated
Automatic update: OMIM entry 604565 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).