Annexin A6 (ANXA6)

The protein contains 673 amino acids for an estimated molecular weight of 75873 Da.

 

May associate with CD21. May regulate the release of Ca(2+) from intracellular stores. (updated: Oct. 10, 2018)

Protein identification was indicated in the following studies:

  1. Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
  2. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete		TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.
Protein sequence (FASTA)
Protein coevolution profile (FreeContact)
Multiple Sequence Alignment (Muscle)

Interpro domains
Total structural coverage: 100%
Model score: 100
No model available.

(right-click above to access to more options from the contextual menu)

The reference OMIM entry for this protein is 114070

Annexin a6; anxa6
Annexin vi; anx6
Calcium-binding protein p68
Calelectrin

DESCRIPTION

ANXA6 is a member of a family of proteins that bind membrane or cytoskeleton in a Ca(2+)-dependent manner. These proteins are characterized by homologous amino acid sequences that are present in multiple copies in each protein. The family is variously known as calelectrins, annexins, calpactins, endonexins, and lipocortins.

CLONING

Three members of the calelectrin family with apparent molecular masses of 67 kD, 35 kD, and 32.5 kD had been purified to homogeneity. Sudhof et al. (1988) reported the cDNA cloning and primary structure of human 67-kD calelectrin. The deduced sequence contains 8 similar repeats, each consisting of about 68 amino acids. Comparison of the 67-kD calelectrin sequence with the protein sequences of lipocortins I and II (151690, 151740) demonstrated a close relationship (42 to 45% identity). Thomas et al. (2002) found that Anxa6 showed a punctate supranuclear and apical distribution in rat pancreatic acinary cells. Anxa6 colocalized with Crhsp28, a component of a protein complex associated with vesicular structures.

MAPPING

Davies et al. (1989) assigned the ANXA6 gene to 5q32-q34 by use of a cDNA clone to probe genomic DNA from rodent-human somatic cell hybrids and for in situ hybridization. The corresponding gene in the mouse was assigned to chromosome 11 by probing DNA from rodent-rodent somatic cell hybrids. Warrington and Bengtsson (1994) used 3 physical mapping methods (radiation hybrid mapping, pulsed field gel electrophoresis, and fluorescence in situ hybridization of interphase nuclei) to determine the order and relative distances between 12 loci in the 5q31-q33 region. ANXA6 was one of those loci.

GENE STRUCTURE

Smith et al. (1994) demonstrated that the ANX6 gene is approximately 60 kb long and contains 26 exons. The genomic sequence at the 3-prime end does not contain a canonical polyadenylylation signal. The genomic sequence upstream of the transcription start site contains TATAA and CAAT motifs. The spatial organization of the exons revealed no obvious similarities between the 2 halves of the ANX6 gene. Comparison of the intron/exon boundary positions of ANX6 with those of ANX1 (151690) and ANX2 (151740) revealed that within the repeated domains the breakpoints are perfectly conserved except for exon 8, which is 1 codon smaller in ANX2. The corresponding point in the second half of ANX6 is represented by 2 exons, exons 20 and 21. The latter exon is alternatively spliced, giving rise to annexin VI isoforms that differ with respect to a 6-amino acid insertion at the start of repeat 7.

GENE FUNCTION

Thomas et al. (2002) found that stimulation of rat pancreatic acinary cells with the secretagogue cholecystokinin (118440) enhanced the colocalization of Anxa6 and Crhsp28 within regions of acini immediately below the apical plasma membrane. Thomas et al. (2002) concluded that Ca(2+)-dependent binding between CRHSP28 and ANXA6 is required for acinar cell membrane trafficking events and digestive enzyme secretion. ... More on the omim web site

Subscribe to this protein entry history

Oct. 19, 2018: Protein entry updated
Automatic update: OMIM entry 114070 was added.

Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).