Carboxypeptidase N subunit 2 (CPN2)
The protein contains 545 amino acids for an estimated molecular weight of 60557 Da.
The 83 kDa subunit binds and stabilizes the catalytic subunit at 37 degrees Celsius and keeps it in circulation. Under some circumstances it may be an allosteric modifier of the catalytic subunit. (updated: Oct. 10, 2018)
Protein identification was indicated in the following studies:
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs3732477 | |
| dbSNP:rs4974538 | |
| dbSNP:rs11711157 |
No binding partner found
The reference OMIM entry for this protein is 603104
Carboxypeptidase n, polypeptide 2, 83-kd; cpn2
Carboxypeptidase n, regulatory subunit
DESCRIPTION
Carboxypeptidase N (arginine carboxypeptidase, EC 3.4.17.3) is a 280-kD tetrameric glycoprotein that is synthesized by the liver and secreted into the plasma. It consists of 2 identical 83-kD regulatory subunits and 2 identical 50-kD catalytic subunits (CPN1; 603103). Carboxypeptidase N has frequently been called serum or plasma carboxypeptidase B, which is misleading since the enzymatic and physical properties and the protein sequences of carboxypeptidase N and pancreatic carboxypeptidase B (114852) are distinct (Tan et al., 1990).CLONING
By screening a liver expression library with antibodies against carboxypeptidase N, Tan et al. (1990) isolated a cDNA encoding the 83-kD regulatory subunit of carboxypeptidase N (CPN2). The predicted 536-amino acid protein contains a region of twelve 24-residue leucine-rich repeats and 5 leucine zipper motifs.MAPPING
By analysis of a cloned breakpoint from a translocation t(3;8)(q27-29;p23) present in a hepatocellular carcinoma, Pineau et al. (1996) determined that a CPN2 pseudogene is located at 8p23-p22. They mapped the CPN2 gene to the same region by in situ hybridization. ... More on the omim web site
June 30, 2020: Protein entry updated
Automatic update: OMIM entry 603104 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).