Phosphatidylinositol-glycan-specific phospholipase D (GPLD1)
The protein contains 840 amino acids for an estimated molecular weight of 92336 Da.
This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane. (updated: Oct. 10, 2018)
Protein identification was indicated in the following studies:
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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No binding partner found
Biological Process
C-terminal protein lipidation
Cell migration involved in sprouting angiogenesis
Cellular response to calcium ion
Cellular response to cholesterol
Cellular response to drug
Cellular response to insulin stimulus
Cellular response to pH
Cellular response to triglyceride
Chondrocyte differentiation
Complement receptor mediated signaling pathway
GPI anchor release
Hematopoietic stem cell migration
Hematopoietic stem cell migration to bone marrow
Insulin receptor signaling pathway
Negative regulation of cell population proliferation
Negative regulation of triglyceride catabolic process
Ossification
Phosphatidylcholine metabolic process
Positive regulation of alkaline phosphatase activity
Positive regulation of apoptotic process
Positive regulation of endothelial cell migration
Positive regulation of glucose metabolic process
Positive regulation of high-density lipoprotein particle clearance
Positive regulation of insulin secretion involved in cellular response to glucose stimulus
Positive regulation of membrane protein ectodomain proteolysis
Positive regulation of secretion
Positive regulation of triglyceride biosynthetic process
Regulation of cellular response to insulin stimulus
Response to glucose
Transepithelial transport
The reference OMIM entry for this protein is 602515
Phospholipase d1, glycosylphosphatidylinositol-specific; gpld1
Phospholipase d, phosphatidylinositol-glycan-specific; pigpld
Glycosylphosphatidylinositol-specific phospholipase d; gpipld
CLONING
Many proteins are attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor. Phosphatidylinositol-glycan (PIG)-specific phospholipases D (PLDs) selectively hydrolyze the inositol phosphate linkage, allowing release of the protein. Scallon et al. (1991) cloned a cDNA encoding a PIGPLD from a bovine liver cDNA library. The deduced amino acid sequence contains 4 regions of internal homology that are similar to the metal ion binding domains of integrin alpha subunits (see ITGA2, 192974). Bovine PIGPLD does not exhibit phosphatidylcholine-specific PLD (602382) activity. By PCR and screening of a human liver cDNA library, Tsang et al. (1992) isolated a cDNA (GENBANK L11701) encoding a PIGPLD. The protein product contains 841 amino acids, including a 24-residue signal sequence. Tsang et al. (1992) isolated a cDNA (GENBANK L11702) encoding a related but distinct PIGPLD from a human pancreas cDNA library. The pancreas-derived PIGPLD contains 840 amino acids, including a 23-residue signal sequence. LeBoeuf et al. (1998) isolated a mouse Gpld1 cDNA. They reported that the predicted mouse and human GPIPLD proteins are 74% identical. By Northern blot analysis, Schofield and Rademacher (2000) showed that expression of a 5.8-kb GPLD1 transcript is restricted to liver. Southern blot analysis indicated that GPLD1 is a single-copy gene.GENE STRUCTURE
Schofield and Rademacher (2000) determined that the GPLD1 gene contains 25 exons and spans at least 80 kb.MAPPING
By analysis of an interspecific backcross, LeBoeuf et al. (1998) mapped the mouse Gpld1 gene to chromosome 13. Using FISH, Schofield and Rademacher (2000) mapped the GPLD1 gene to 6p22.1. ... More on the omim web site
June 30, 2020: Protein entry updated
Automatic update: OMIM entry 602515 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).