Hepatocyte growth factor activator (HGFAC)
The protein contains 655 amino acids for an estimated molecular weight of 70682 Da.
Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form. (updated: Oct. 10, 2018)
Protein identification was indicated in the following studies:
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs3748034 | |
| dbSNP:rs16844370 | |
| dbSNP:rs1987546 | |
| dbSNP:rs16844401 | |
| dbSNP:rs2498323 |
No binding partner found
The reference OMIM entry for this protein is 604552
Hepatocyte growth factor activator; hgfac
Hgf activator; hgfa
CLONING
Hepatocyte growth factor (HGF; 142409) is a potent mitogen for parenchymal liver cells, epithelial cells, and endothelial cells. It is first synthesized as an inactive single-chain precursor before being activated to a heterodimeric form by endoproteolytic processing. Shimomura et al. (1992) purified from bovine serum a 34-kD serine protease responsible for this activation. Miyazawa et al. (1993) determined the partial amino acid sequence of the homologous human serine protease and used it to design degenerate primers to screen a human liver cDNA library. They identified a 655-amino acid inactive precursor protein, designated hepatocyte growth factor activator (HGFAC), with a calculated molecular mass of 70,681 Da. They determined that the active protein purified from serum is derived from the C terminus of the precursor by proteolytic cleavage of the bonds between arg372-val373 and arg407-ile408. The precursor has about 39% similarity with blood coagulation factor XII (610619), which circulates in plasma as an inactive zymogen. Shimomura et al. (1993) determined that the serum form of the HGFAC precursor is a 96- to 98-kD protein that can be activated to a 2-chain form of 66 and 32 kD by thrombin in the presence of dextran sulfate by cleavage at the arg407-ile408 bond. The proteolytically cleaved form activates single-chain HGF. By Northern blot analysis, Miyazawa et al. (1993) identified HGFAC transcripts of 3.5 and 2.2 kb in liver but not in lung, kidney, leukocytes, or placenta.GENE STRUCTURE
Miyazawa et al. (1998) determined the complete sequence of the HGFAC gene, which spans about 7.5 kb of DNA and consists of 14 exons separated by 13 introns. Sequence analysis revealed that factor XII and HGFAC share multiple structural homologies, suggesting that they arose through gene duplication.MAPPING
Miyazawa et al. (1998) mapped the HGFAC gene to chromosome 4p16, using spot-blot hybridization on sorted chromosomes and fluorescence in situ hybridization on metaphase chromosome spreads. ... More on the omim web site
Oct. 20, 2018: Protein entry updated
Automatic update: OMIM entry 604552 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).