Inter-alpha-trypsin inhibitor heavy chain H3 (ITIH3)
The protein contains 890 amino acids for an estimated molecular weight of 99849 Da.
May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes. (updated: Oct. 10, 2018)
Protein identification was indicated in the following studies:
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs3617 | |
| dbSNP:rs35271262 | |
| dbSNP:rs60805548 | |
| dbSNP:rs9883888 | |
| dbSNP:rs2710330 | |
| dbSNP:rs2710329 |
No binding partner found
The reference OMIM entry for this protein is 146650
Inter-alpha-trypsin inhibitor, heavy chain 3; itih3
DESCRIPTION
The inter-alpha-trypsin inhibitors (ITI) are a family of structurally related plasma serine protease inhibitors involved in extracellular matrix stabilization and in prevention of tumor metastasis. The ITI family contains multiple proteins made up of a light chain (see 176870) and a variable number of heavy chains (Salier et al., 1987; Himmelfarb et al., 2004).CLONING
By screening a liver expression library with antibodies against ITI chains, Salier et al. (1987) isolated several partial cDNAs encoding ITI heavy chains. Diarra-Mehrpour et al. (1989) analyzed the in vitro translation products of liver mRNAs hybrid-selected by these partial cDNAs and determined that they encoded 3 different heavy chains: H1 (ITIH1; 147270), H2 (ITIH2; 146640) and H3 (ITIH3). Enghild et al. (1989) found that one ITI member, the pre-alpha-trypsin inhibitor (P-alpha-I), is an approximately 130-kD protein that contains the mature ITIH3 heavy chain (HC3) and a 30-kD subunit. Bourguignon et al. (1993) stated that P-alpha-I is composed of HC3 covalently linked to bikunin (see AMBP, 176870), a Kunitz-type protease inhibitor. They reported that the 885-amino acid H3 precursor protein contains a 10-amino acid propeptide and a 19-amino acid signal peptide. Before or during linkage to bikunin, the C-terminal 238 amino acids are cleaved to produce mature HC3. H3 shares 54% and 41% identity with the H1 and H2 precursor proteins, respectively. Diarra-Mehrpour et al. (1998) corrected the cDNA sequence reported by Bourguignon et al. (1993).GENE STRUCTURE
Diarra-Mehrpour et al. (1998) determined that, like ITIH1, the ITIH3 gene contains 22 exons. The intron/exon boundaries occur at the same positions in both genes.MAPPING
By in situ hybridization, Diarra-Mehrpour et al. (1989) mapped the ITIH3 gene to 3p21.2-p21.1. Diarra-Mehrpour et al. (1998) reported that ITIH3 is located within a cluster of 3 ITIH genes that spans 45 kb. The genes are arranged ITIH1--ITIH3--ITIH4 (600564). The ITIH4 gene is transcribed in the opposite orientation. ... More on the omim web site
Oct. 20, 2018: Protein entry updated
Automatic update: OMIM entry 146650 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).