Keratin, type I cytoskeletal 27 (KRT27)
The protein contains 459 amino acids for an estimated molecular weight of 49822 Da.
Essential for the proper assembly of type I and type II keratin protein complexes and formation of keratin intermediate filaments in the inner root sheath (irs). (updated: Sept. 12, 2018)
Protein identification was indicated in the following studies:
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs2469826 | |
| dbSNP:rs12453124 | |
| dbSNP:rs17558560 | |
| dbSNP:rs17558532 | |
| dbSNP:rs981684 |
No binding partner found
The reference OMIM entry for this protein is 616676
Keratin 27, type i; krt27
K27
Krt25c; k25c
Ka40
K10b
Keratin 25, inner root sheath, 3; krt25irs3; k25irs3
Irsa3.1
Cytokeratin 27; ck27
DESCRIPTION
Keratins are structural proteins that form the intermediate filament cytoskeleton in all epithelia. They assemble into long filaments built from heterodimeric double-stranded coils of type I and type II keratins. KRT27 is a type I keratin of hair inner root sheath Hesse et al. (2004).CLONING
By database analysis and RT-PCR of a human scalp cDNA library, Rogers et al. (2004) cloned KRT27, which they called K25IRS3. The deduced 459-amino acid protein has a calculated molecular mass of 49.8 kD and contains a long central alpha-helical region. RT-PCR detected K25IRS3 expression in skin and scalp, but not in tongue, thymus, breast, placenta, or eye. By in situ hybridization and immunohistochemical analysis of human hair follicle, Langbein et al. (2006) found that K27 was expressed in the Henle layer, the Huxley layer, and the inner root sheath cuticle. Expression extended from the bulb region to the points of terminal differentiation in these 3 layers. K27 was also expressed in hair medulla.GENE STRUCTURE
Rogers et al. (2004) reported that the KRT27 gene has 8 exons.MAPPING
By genomic sequence analysis, Hesse et al. (2004) mapped the KRT27 gene to the keratin I gene cluster on chromosome 17q21.2. The mouse Krt27 gene is located in a keratin I cluster in a region of chromosome 11D that shares homology of synteny with human chromosome 17q21.NOMENCLATURE
Schweizer et al. (2006) provided an updated nomenclature for keratins. They noted that KRT27 had previously been called K25IRS3, K10B, IRSA3.1, KRT25C, and KA40. ... More on the omim web site
June 30, 2020: Protein entry updated
Automatic update: OMIM entry 616676 was added.
Feb. 23, 2019: Protein entry updated
Automatic update: model status changed
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).