Dynamin-3 (DNM3)

The protein contains 869 amino acids for an estimated molecular weight of 97746 Da.

 

Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis (By similarity). (updated: Oct. 10, 2018)

Protein identification was indicated in the following studies:

  1. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
  2. Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete		TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.
Protein sequence (FASTA)
Protein coevolution profile (FreeContact)
Multiple Sequence Alignment (Muscle)

This protein is annotated as membranous in Gene Ontology.


Interpro domains
Total structural coverage: 0%
Model score: 0
No model available.

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The reference OMIM entry for this protein is 611445

Dynamin 3; dnm3
Kiaa0820

DESCRIPTION

Members of the dynamin family, such as DNM3, possess mechanochemical properties involved in actin-membrane processes, predominantly in membrane budding (Orth and McNiven, 2003).

CLONING

By sequencing clones obtained from a size-fractionated brain cDNA library, Nagase et al. (1998) cloned DNM3, which they designated KIAA0820. The deduced protein contains 702 amino acids. RT-PCR ELISA detected very high expression in adult brain and spinal cord and in all specific brain regions examined. Intermediate expression was detected in adult heart, lung, kidney, testis, and ovary and in fetal liver and brain. Low expression was detected in adult liver and pancreas, and little to none was detected in skeletal muscle and spleen.

GENE FUNCTION

Lu et al. (2007) noted that dynamin-3 localizes to postsynaptic spines and binds the postsynaptic adaptor protein Homer (see 604798) (Gray et al., 2003), potentially linking dynamin-3 to the postsynaptic density (PSD) scaffold. Using a combination of live-cell imaging and quantitative light and electron microscopy of rat hippocampal neurons, Lu et al. (2007) demonstrated that dynamin-3 positioned the clathrin (see 118955) endocytic machinery near the PSD through its interaction with the Homer-Shank (see 604999) scaffold complex. Disruption of dynamin-3 uncoupled the PSD from the endocytic zone, leading to loss of synaptic Glur1 (GRIA1; 138248) AMPA receptors, and depressed excitatory synaptic transmission due to failure of endocytosed receptors to locally recycle back to the synapse. Scott et al. (2012) found that intron 13 of the DNM3 gene in rat and human harbors 2 microRNAs (miRNAs), MIR3120 (614722) and MIR214 (610721). MIR3120 is encoded on the same strand as DNM3, and MIR214 overlaps MIR3120 on the opposite strand and is expressed from a different promoter. Northern blot analysis showed that Dnm3, Mir3120, and Mir214 were coexpressed in cultured rat hippocampal neurons. Like Dnm3, Mir3120 appeared to regulate expression of proteins involved in endocytosis of clathrin-coated vesicles.

GENE STRUCTURE

In their Figure 1, Scott et al. (2012) showed that the DNM3 gene contains at least 18 exons. They found that intron 13 harbors the MIR3120 gene in the sense orientation and MIR214, which overlaps MIR1320, in the antisense orientation.

MAPPING

Hartz (2012) mapped the DNM3 gene to chromosome 1q24.3 based on an alignment of the DNM3 sequence (GenBank GENBANK AB020627) with the genomic sequence (GRCh37). ... More on the omim web site

Subscribe to this protein entry history

Nov. 17, 2018: Protein entry updated
Automatic update: OMIM entry 611445 was added.

Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).