Myosin-4 (MYH4)
The protein contains 1939 amino acids for an estimated molecular weight of 223071 Da.
Muscle contraction. (updated: Oct. 10, 2018)
Protein identification was indicated in the following studies:
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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No binding partner found
The reference OMIM entry for this protein is 160742
Myosin, heavy chain 4, skeletal muscle; myh4
Myosin, skeletal muscle, heavy chain
Myosin, heavy chain, iib; myhc2b; myh2b
Myhc iib
Mhc iib
For background information on the myosin heavy chain genes, see MYH1 (160730).
CLONING
Edwards et al. (1985) isolated a cDNA clone complementary to the mRNA encoding human myosin heavy chain from a fetal skeletal muscle cDNA library. By RT-PCR of human skeletal muscle RNA, Weiss et al. (1999) cloned full-length MHC IIb. All MHC proteins share approximately 100% conservation in the phosphate-binding loop, several helices forming the nucleotide-binding pocket, residues involved in actin binding, residues involved in the stereospecific hydrophobic rigor state interaction of actin with myosin, a cleft that divides the ATP- and actin-binding sites, and a converter domain, or fulcrum, containing 2 conserved cysteines.MAPPING
Edwards et al. (1985) used a 600-bp fragment of an MYH4 cDNA as a probe in Southern analysis of DNA from panels of rat/human and mouse/human somatic cell hybrids. All sequences detected by this probe mapped to 17pter-p11. Soussi-Yanicostas et al. (1993) presented evidence that at least 5 skeletal myosin heavy chain genes are clustered in a 320-kb genomic segment. Thus, the MYH4 gene would appear to be located on 17p13.1, the location of 4 other myosin heavy chain genes (MYH1; MYH2, 160740; MYH3, 160720; and MYH8, 160741). Furthermore, Soussi-Yanicostas et al. (1993) had evidence from analysis of YACs that the embryonic and fetal genes, on the one hand, and the adult fast myosin heavy chain genes, on the other hand, are contained within different genomic fragments; see 160730. They suggested that the genes encoding the 2 developmental forms are adjacent in the human genome and that temporal regulation of the myosin heavy chain genes may be related to their organization within the cluster. ... More on the omim web site
Oct. 20, 2018: Protein entry updated
Automatic update: OMIM entry 160742 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).