Cyclin-Y (CCNY)
The protein contains 341 amino acids for an estimated molecular weight of 39337 Da.
Positive regulatory subunit of the cyclin-dependent kinases CDK14/PFTK1 and CDK16. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by recruiting CDK14/PFTK1 to the plasma membrane and promoting phosphorylation of LRP6, leading to the activation of the Wnt signaling pathway. Recruits CDK16 to the plasma membrane. Isoform 3 might play a role in the activation of MYC-mediated transcription. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology, is annotated as membranous in UniProt.
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Biological Process
Cell division
G2/M transition of mitotic cell cycle
Positive regulation of cyclin-dependent protein serine/threonine kinase activity
Regulation of canonical Wnt signaling pathway
Wnt signaling pathway
The reference OMIM entry for this protein is 612786
Cyclin y; ccny
Cyclin-fold protein 1; cfp1
Cyclin x; ccnx
Chromosome 10 open reading frame 9; c10orf9
DESCRIPTION
Cyclins, such as CCNY, control cell division cycles and regulate cyclin-dependent kinases (e.g., CDC2; 116940) (Li et al., 2009).CLONING
Using mass spectrometry to identify proteins upregulated in metastatic versus nonmetastatic human colorectal adenocarcinoma cell lines, followed by database analysis, Zhang et al. (2005) identified an isoform of CCNY that they called CFP1 variant A. The deduced protein has a calculated molecular mass of 36.9 kD. By database analysis and PCR of a testis cDNA library, Li et al. (2009) cloned CCNY, which they designated CCNX. The deduced 287-amino acid protein has a central cyclin box and shares highest similarity with cyclin B3 (CCNB3; 300456). RT-PCR detected highest expression in testis and lower expression in all other tissues examined except brain and kidney. Fluorescence-tagged CCNX was expressed predominantly in the nucleus in a human nonsmall lung cancer cell line.GENE STRUCTURE
Li et al. (2009) determined that the CCNY gene contains 12 exons.MAPPING
By genomic sequence analysis, Deloukas et al. (2004) mapped the CCNY gene to chromosome 10p11.2, where it lies between the CREM (123812) and FZD8 (606146) genes.GENE FUNCTION
Using the promoter regions of several cell cycle regulators and transcription factors, Li et al. (2009) showed that CCNX specifically activated transcription from the MYC (190080) promoter.MOLECULAR GENETICS
For discussion of a possible association between variation in the CCNY gene and inflammatory bowel disease, see IBD1 (266600). ... More on the omim web site
Dec. 10, 2018: Protein entry updated
Automatic update: model status changed
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 612786 was added.