Alpha-1-acid glycoprotein 2 (ORM2)

The protein contains 201 amino acids for an estimated molecular weight of 23603 Da.

 

Functions as transport protein in the blood stream. Binds various hydrophobic ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability. Appears to function in modulating the activity of the immune system during the acute-phase reaction. (updated: Jan. 7, 2015)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  3. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete		TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.
Protein sequence (FASTA)
Protein coevolution profile (FreeContact)
Multiple Sequence Alignment (Muscle)

Interpro domains
Total structural coverage: 92%
Model score: 0
No model available.

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VariantDescription
dbSNP:rs147969317
dbSNP:rs2636889
dbSNP:rs12685968
dbSNP:rs1126777
dbSNP:rs2636890

The reference OMIM entry for this protein is 138610

Orosomucoid 2; orm2
Glycoprotein, alpha-1-acid, of serum, type 2
Alpha-1-acid glycoprotein, type 2; agp2

CLONING

Yuasa et al. (1986) performed orosomucoid phenotyping by isoelectric focusing and immunoprinting. The band pattern of desialyzed ORM indicated to these workers that the ORM system is controlled by 2 structural loci, ORM1 (138600) and ORM2. By studies of orosomucoid cDNA clones isolated from mouse liver, Cooper and Papaconstantinou (1986) showed that 2 species of ORM mRNA are transcribed from 2 distinct genes. Escallon et al. (1987) presented evidence for the expression of a second structural locus (ORM2) in human plasma as well as for the detection of genetically determined variation at the ORM2 locus in American blacks by the use of a sensitive enzyme-linked immunoblotting technique. After thin-layer polyacrylamide isoelectric focusing (IEF) in gels containing 6 M urea, the isoprotein species coded by the second locus did not segregate with the predominant alleles at the ORM1 locus. Variation in ORM2 was not observed in U.S. whites or in several other populations studied. Because of the low frequency and unlimited distribution of ORM2 variation, Escallon et al. (1987) were unable to test for linkage between the 2 ORM loci. Yuasa et al. (1997) found that the ORM1 and ORM2 genes encode 183-amino acid polypeptides. Merritt and Board (1988) found at least 21 amino acid differences between the products of the 2 genes.

GENE STRUCTURE

Yuasa et al. (1997) found that the ORM1 and ORM2 genes contain 6 exons. Merritt and Board (1988) presented the complete nucleotide sequence of the ORM2 gene.

MAPPING

By in situ hybridization using a cDNA clone for AGP2, Webb et al. (1988) mapped both alpha-1-acid glycoprotein genes in the 9q34 band. Yuasa et al. (1988) demonstrated linkage disequilibrium between alleles at the ORM1 and ORM2 loci, thus corroborating the close linkage demonstrated physically by Dente et al. (1987). Merritt and Board (1988) found that the ORM2 gene is located approximately 3.3 kb downstream from ORM1.

MOLECULAR GENETICS

Data on gene frequencies of allelic variants were tabulated by Roychoudhury and Nei (1988). Yuasa et al. (1987) demonstrated polymorphism of the ORM2 locus, with 6 alleles in the Japanese population. Yuasa et al. (1988) used a modified isoelectric focusing, in which glycerine was omitted from gels, to differentiate ORM1 and ORM2 variants. Using a new method for ORM phenotyping, Eap et al. (1988) discovered a rare variant tentatively assigned to the ORM2 locus and a common variant tentatively assigned to the ORM1 locus. Umetsu et al. (1988) reported on polymorphism of ORM1 and ORM2 in a population in the Philippines. ORM2 was nonpolymorphic, i.e., monomorphic, in that population when studied by that method. ... More on the omim web site

Subscribe to this protein entry history

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated

March 25, 2017: Additional information
No protein expression data in P. Mayeux work for ORM2

March 16, 2016: Protein entry updated
Automatic update: OMIM entry 138610 was added.

Jan. 27, 2016: Protein entry updated
Automatic update: model status changed

Jan. 24, 2016: Protein entry updated
Automatic update: model status changed