Serpin B3 (SERPINB3)
The protein contains 390 amino acids for an estimated molecular weight of 44565 Da.
May act as a papain-like cysteine protease inhibitor to modulate the host immune response against tumor cells. Also functions as an inhibitor of UV-induced apoptosis via suppression of the activity of c-Jun NH(2)-terminal kinase (JNK1). (updated: April 1, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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Biological Process
Autocrine signaling
Negative regulation of catalytic activity
Negative regulation of endopeptidase activity
Negative regulation of JUN kinase activity
Negative regulation of peptidase activity
Negative regulation of proteolysis
Neutrophil degranulation
Paracrine signaling
Positive regulation of cell migration
Positive regulation of cell population proliferation
Positive regulation of endopeptidase activity
Positive regulation of epithelial to mesenchymal transition
Viral entry into host cell
The reference OMIM entry for this protein is 600517
Serpin peptidase inhibitor, clade b (ovalbumin), member 3; serpinb3
Squamous cell carcinoma antigen 1; scca1
DESCRIPTION
Squamous cell carcinoma antigen (SCCA) is a member of the ovalbumin family of serine proteinase inhibitors. The protein was isolated from a metastatic cervical squamous cell carcinoma by Kato and Torigoe (1977). SCCA is detected in the superficial and intermediate layers of normal squamous epithelium, whereas the mRNA is detected in the basal and subbasal levels. The clinical import of SCCA has been as a circulating tumor marker for squamous cell carcinoma, especially those of the cervix, head and neck, lung, and esophagus. Many clinical studies of cervical squamous cell carcinoma show that the percentage of patients with elevated circulating levels of SCCA increases from approximately 12% at stage 0 to more than 90% at stage IV. Levels fall after tumor resection and rise in approximately 90% of the patients with recurrent disease. Similar trends occur in the other types of squamous cell carcinoma, with a maximum sensitivity of approximately 60% for lung, 50% for esophageal, and 55% for head and neck tumors. The neutral form of SCCA (SCCA1, or SERPINB3) is detected in the cytoplasm of normal and some malignant squamous cells, whereas the acidic form (SCCA2, or SERPINB4; 600518) is expressed primarily in malignant cells and is the major form found in the plasma of cancer patients. Thus, the appearance of the acidic fraction of SCCA is correlated with more aggressive tumors (summary by Schneider et al., 1995).CLONING
Suminami et al. (1991) cloned the cDNA for SCCA. The deduced protein contains 390 amino acids and belongs to the serine protease inhibitor family. Northern blot analysis detected a transcript of about 1.7 kb in human squamous cells. Based on the predicted pI values and molecular masses of SCCA1 and SCCA2, Schneider et al. (1995) suggested that the neutral and acidic forms of SCCA are encoded by SCCA1 and SCCA2, respectively. SCCA1 shares 92% amino acid identity with SCCA2.GENE FUNCTION
Ray et al. (2005) stated that mouse Scca2, or Serpinb3a, exhibits the cysteine-like serine protease inhibitory function of human SCCA1 and the trypsin-like serine protease inhibitory function of human SCCA2. Thus, Scca2 is the mouse ortholog of both human SCCA1 and SCCA2. Ray et al. (2005) found that challenge of uteroglobin (UGB, or SCGB1A1; 192020)-knockout mice with the allergen chicken ovalbumin (OVA) resulted in elevated lung expression of Scca2, as well as elevated levels of the cytokines Il4 (147780) and Il13 (147683) in lung and exacerbated airway inflammation. These effects were countered by reintroduction of recombinant Ugb. Treatment of cultured human bronchial epithelial cells with IL4 or IL13 stimulated SCCA1 and SCCA2 expression via phosphorylation of the transcription factors STAT1 (600555) and STAT6 (601512). SCCA1 and SCCA2 expression was not upregulated by IL4 or IL13 in the presence of an inhibitor of tyrosine phosphorylation. Ray et al. (2005) proposed that UGB controls allergic asthma by downregulating signaling through IL4 and IL13 and inhibiting SCCA1 and SCCA2 expression.MAPPING
By fluorescence in situ hybridization, Kuwano et al. (1995) mapped the SCCA1 gene to chromosome 18q21. They noted that the PLANH2 gene (173390), with which the SCCA1 gene shares much homology, also maps to chromosome 18q21. The oncogene BCL2 (151430), which is located 600 kb centromeric to PLAH2, is also located on chromosome 18q21. Schneider et al. (1995) found that SCCA1 and SCCA2 are t ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 25, 2017: Additional information
No protein expression data in P. Mayeux work for SERPINB3
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 600517 was added.
Jan. 28, 2016: Protein entry updated
Automatic update: model status changed
Jan. 25, 2016: Protein entry updated
Automatic update: model status changed