60S ribosomal protein L9 (RPL9P9)
The protein contains 192 amino acids for an estimated molecular weight of 21863 Da.
No function (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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Biological Process
Cellular protein metabolic process
Cytoplasmic translation
Gene expression
Nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
RRNA processing
SRP-dependent cotranslational protein targeting to membrane
Translation
Translational elongation
Translational initiation
Translational termination
Viral life cycle
Viral process
Viral transcription
The reference OMIM entry for this protein is 603686
Ribosomal protein l9; rpl9
The eukaryotic ribosome, which consists of a large (60S) subunit and a small (40S) subunit, is composed of 4 RNA species (see 180450) and approximately 80 different proteins. The ribosomal protein genes appear to be members of multigene families, most of which are composed of multiple intronless pseudogenes and 1 intron-containing functional gene. Hori et al. (1993) isolated a cDNA encoding the 60S ribosomal protein L9 (RPL9) from a human liver cancer cell line (HepG2) cDNA library. The deduced 192-amino acid human RPL9 protein differs from the rat Rpl9 protein by 2 amino acids. Northern blot analysis detected an approximately 800-bp RPL9 transcript in HepG2 cells. Mazuruk et al. (1996) isolated RPL9 cDNAs and genomic clones. The RPL9 gene contains 8 exons and spans approximately 5.5 kb. Northern blot analysis showed expression of an approximately 700-bp RPL9 transcript in all human adult and fetal tissues examined. The authors detected at least 8 putative processed RPL9 pseudogenes. Using somatic cell hybrid analysis and FISH, Mazuruk et al. (1996) mapped the intron-containing RPL9 gene to 4p13. Kenmochi et al. (1998) confirmed the RPL9 mapping assignment reported by Mazuruk et al. (1996). Mazuruk et al. (1996) mapped an RPL9 pseudogene (GenBank GENBANK U09955) to Xpter-p21. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 25, 2017: Additional information
No protein expression data in P. Mayeux work for RPL9P9
March 15, 2016: Protein entry updated
Automatic update: OMIM entry 603686 was added.