Pirin (PIR)
The protein contains 290 amino acids for an estimated molecular weight of 32113 Da.
Transcriptional coregulator of NF-kappa-B which facilitates binding of NF-kappa-B proteins to target kappa-B genes in a redox-state-dependent manner. May be required for efficient terminal myeloid maturation of hematopoietic cells. Has quercetin 2,3-dioxygenase activity (in vitro). (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs34104000 |
The reference OMIM entry for this protein is 300931
Pirin; pir
CLONING
NFI/CTF1 (see 164005) is a DNA-binding protein that stimulates adenovirus replication and RNA polymerase II-mediated transcription. Using a yeast 2-hybrid screen with NFI/CTF1 as bait, Wendler et al. (1997) isolated partial cDNAs encoding pirin. By screening a HeLa cell library with a partial pirin cDNA, the authors recovered additional cDNAs representing alternatively spliced transcripts that differed in their 5-prime UTRs but encoded the same 290-amino acid protein. Immunofluorescence studies showed that pirin localized in dot-like structures in nuclei of mammalian cells. Antibodies against pirin recognized a single band in extracts of cells from several mammals, suggesting that pirin is conserved among mammals. Northern blot analysis revealed that pirin was expressed as an approximately 1.5-kb mRNA in all tissues tested, with highest levels in heart and liver. Additional larger transcripts were detected at low levels in some tissues.MAPPING
Gross (2014) mapped the PIR gene to chromosome Xp22.2 based on an alignment of the PIR sequence (GenBank GENBANK BC002517) with the genomic sequence (GRCh38). ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 300931 was added.
Jan. 28, 2016: Protein entry updated
Automatic update: model status changed
Jan. 25, 2016: Protein entry updated
Automatic update: model status changed