Annexin A11 (ANXA11)
The protein contains 505 amino acids for an estimated molecular weight of 54390 Da.
Binds specifically to calcyclin in a calcium-dependent manner (By similarity). Required for midbody formation and completion of the terminal phase of cytokinesis. (updated: April 1, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
(right-click above to access to more options from the contextual menu)
| Variant | Description |
|---|---|
| dbSNP:rs2229554 | |
| dbSNP:rs1049550 | |
| dbSNP:rs1802932 | |
| ALS23 | |
| ALS23 | |
| ALS23 | |
| ALS23 | |
| ALS23 | |
| ALS23 |
Biological Process
Cell cycle
Cell division
Cytokinetic process
Phagocytosis
Response to calcium ion
The reference OMIM entry for this protein is 602572
Annexin a11; anxa11
Annexin xi; anx11
Autoantigen, 56-kd
DESCRIPTION
ANXA11 is a member of the annexin family of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and homologous C-terminal domains containing the calcium-dependent phospholipid-binding sites.CLONING
To identify a 56-kD antigen recognized by sera from patients with autoimmune diseases, Misaki et al. (1994) screened a human teratocarcinoma cDNA expression library with the anti-56-kD antigen autoantibodies. They isolated a cDNA predicting a 505-amino acid protein with 60% identity to other annexins in the conserved C-terminal domain and 92.5% identity to bovine annexin XI over the entire protein. Misaki et al. (1994) concluded that the 56-kD autoantigen is human annexin XI, or ANXA11.GENE STRUCTURE
Bances et al. (2000) determined that the mouse Anxa11 gene contains 15 exons and spans 40 kB. Exon 1 is untranslated, and intron 1 spans about 20 kb. The human ANXA11 gene has the same general structure as the mouse gene; however, EST database analysis indicated that human ANXA11 transcripts also use variably spliced exons 1b and 1c, which can lengthen the 5-prime UTR. The intronic sequences of the mouse and human ANXA11 genes contain several types of repetitive elements, including retroviral long terminal repeats. The mouse promoter region contains a remnant LINE-1 insertion not found in the human gene. The 5-prime flanking region of the ANXA11 gene contains a CpG island and MYOD (159970), SP1 (189906), and glucocorticoid response elements, but no proximal TATA or CAAT boxes.MOLECULAR GENETICS
For discussion of a possible association between variation in the ANXA11 gene and susceptibility to sarcoidosis, see SS3 (612388).MAPPING
Morgan et al. (1998) mapped the ANXA11 gene to 10q22.3-q23.1 by fluorescence in situ hybridization. By genomic sequence analysis, Bances et al. (2000) mapped the mouse Anxa11 gene to chromosome 14. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 602572 was added.