Programmed cell death protein 5 (PDCD5)
The protein contains 125 amino acids for an estimated molecular weight of 14285 Da.
May function in the process of apoptosis. (updated: April 1, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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Biological Process
Apoptotic process
Cellular response to transforming growth factor beta stimulus
Negative regulation of cell population proliferation
Negative regulation of chaperone-mediated protein folding
Positive regulation of apoptotic process
Positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
Positive regulation of gene expression
Positive regulation of protein insertion into mitochondrial outer membrane
Positive regulation of release of cytochrome c from mitochondria
Molecular Function
Acetyltransferase activator activity
Beta-tubulin binding
DNA binding
Heparin binding
The reference OMIM entry for this protein is 604583
Programmed cell death 5; pdcd5
Tf1 cell apoptosis-related gene 19; tfar19
CLONING
Using cDNA-representative differences analysis (Lisitsyn et al., 1993), Liu et al. (1999) identified a novel gene, designated TFAR19, from TF1 cells (a human premyeloid cell line established from a patient with erythroleukemia) undergoing apoptosis after the withdrawal of GM-CSF from the culture medium. The TFAR19 gene encodes a protein of 125 amino acids, with an ATG initiation codon at nucleotides 25-27. It contains a cAMP- and cGMP-dependent protein kinase phosphorylation site, multiple protein kinase C phosphorylation sites, and a casein kinase II phosphorylation site. It shares significant homology to the corresponding proteins of species ranging from yeast to mice. The TFAR19 protein product is predicted to localize in the nucleus. Dot blot analysis showed that TFAR19 is ubiquitously expressed, but is most prominent in heart, testis, kidney, pituitary gland, adrenal gland, and placenta. Expression in fetal tissues is significantly lower than that in adult tissues. The expression of TFAR19 is upregulated in tumor cells undergoing apoptosis and enhances apoptosis triggered by growth factor or serum deprivation.MAPPING
Gross (2014) mapped the PDCD5 gene to chromosome 19q13.11 based on an alignment of the PCDC5 sequence (GenBank GENBANK AF014955) with the genomic sequence (GRCh37). ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
June 20, 2017: Protein entry updated
Automatic update: comparative model was added.
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 604583 was added.
Jan. 28, 2016: Protein entry updated
Automatic update: model status changed
Jan. 25, 2016: Protein entry updated
Automatic update: model status changed