60S ribosomal protein L27 (RPL27)
The protein contains 136 amino acids for an estimated molecular weight of 15798 Da.
Component of the large ribosomal subunit (PubMed:12962325, PubMed:23636399, PubMed:25957688, PubMed:25901680). Required for proper rRNA processing and maturation of 28S and 5.8S rRNAs (PubMed:25424902). (updated: March 28, 2018)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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Biological Process
Cellular protein metabolic process
Cytoplasmic translation
Gene expression
Nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
Response to aldosterone
RRNA processing
SRP-dependent cotranslational protein targeting to membrane
Translation
Translational elongation
Translational initiation
Translational termination
Viral life cycle
Viral process
Viral transcription
Cellular Component
Cytoplasmic side of rough endoplasmic reticulum membrane
Cytosol
Cytosolic large ribosomal subunit
Cytosolic ribosome
Extracellular exosome
Extracellular matrix
Focal adhesion
Intracellular ribonucleoprotein complex
Membrane
Nucleolus
Nucleoplasm
Nucleus
Ribonucleoprotein complex
Ribosome
The reference OMIM entry for this protein is 607526
Ribosomal protein l27; rpl27
CLONING
By differential screening of fetal and adult kidney mRNA, Gallagher et al. (1994) cloned RPL27 as 1 of several transcripts showing high expression during fetal development. The deduced 135-amino acid protein has a calculated molecular mass of about 18 kD. RPL27 shares 100% amino acid identity with rat and chicken Rpl27, although there are several silent base pair changes at the DNA level. Northern blot analysis revealed expression of a 1.0-kb transcript or 2 transcripts of 1.0 and 1.25 kb in fetal kidney. The 1.0-kb transcript was present at lower levels in adult kidney. RPL27 expression was also detected in several other fetal tissues, including muscle, liver, lung, heart, and brain. The 1.0-kb transcript was present in adult muscle at lower levels than in fetal muscle. Gallagher et al. (1994) determined that RPL27 was downregulated 20-fold in adult kidney compared with fetal kidney.GENE FUNCTION
Using RT-PCR, Sim et al. (2010) found that a subset of genes encoding proteins of the large ribosomal subunit was differentially expressed in nasopharyngeal carcinoma (see 607107) cell lines compared with a normal nasopharyngeal epithelial cell line. RPL27 was 1 of 3 genes significantly downregulated in the carcinoma cell lines.MAPPING
By Southern blot analysis, Gallagher et al. (1994) determined that there are multiple RPL27 copies in the genome. The International Radiation Hybrid Mapping Consortium mapped the RPL27 gene to chromosome 17 (TMAP RH26918). ... More on the omim web site
April 12, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 5, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 15, 2016: Protein entry updated
Automatic update: OMIM entry 607526 was added.