Malectin (MLEC)
The protein contains 292 amino acids for an estimated molecular weight of 32234 Da.
Carbohydrate-binding protein with a strong ligand preference for Glc2-N-glycan. May play a role in the early steps of protein N-glycosylation (By similarity). (updated: April 1, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology, is predicted to be membranous by TOPCONS.
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Biological Process
Carbohydrate metabolic process
Cellular protein metabolic process
Neutrophil degranulation
Post-translational protein modification
Protein folding
Protein N-linked glycosylation via asparagine
The reference OMIM entry for this protein is 613802
Malectin; mlec
Kiaa0152
CLONING
By sequencing clones obtained from a size-fractionated KG-1 immature myeloid leukemia cell line cDNA library, Nagase et al. (1995) obtained an MLEC clone, which they designated KIAA0152. The deduced 292-amino acid protein contains a transmembrane domain. Northern blot analysis detected MLEC expression in all human tissues and cell lines examined, with highest expression in heart and skeletal muscle. Schallus et al. (2008) cloned Xenopus Mlec, and they identified human MLEC by database analysis. Both proteins contain an N-terminal signal peptide, a globular domain of about 190 amino acids, and a transmembrane domain near the C terminus. Database analysis revealed Mlec orthologs in most animals, with weaker conservation in plants. Xenopus Mlec was expressed in the endoplasmic reticulum of transfected human osteosarcoma cells.GENE FUNCTION
Schallus et al. (2008) showed that Xenopus Mlec bound specifically to diglucosylated N-glycan, a conserved intermediate in the biosynthesis of N-glycoproteins in the endoplasmic reticulum.MAPPING
By PCR of a human/rodent hybrid panel, Nagase et al. (1995) mapped the MLEC gene to chromosome 12. Hartz (2011) mapped the MLEC gene to chromosome 12q24.31 based on an alignment of the MLEC sequence (GenBank GENBANK BC00037) with the genomic sequence (GRCh37). ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
June 20, 2017: Protein entry updated
Automatic update: comparative model was added.
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 613802 was added.