Mitochondrial enolase superfamily member 1 (ENOSF1)
The protein contains 443 amino acids for an estimated molecular weight of 49786 Da.
Plays a role in the catabolism of L-fucose, a sugar that is part of the carbohydrates that are attached to cellular glycoproteins. Catalyzes the dehydration of L-fuconate to 2-keto-3-deoxy-L-fuconate by the abstraction of the 2-proton to generate an enediolate intermediate that is stabilized by the magnesium ion (PubMed:24697329). (updated: April 1, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs34724061 | |
| dbSNP:rs2612086 | |
| dbSNP:rs2847620 |
No binding partner found
The reference OMIM entry for this protein is 607427
Enolase superfamily member 1; enosf1
Thymidylate synthase, antisense; rts
CLONING
By RT-PCR of KB cells (a human oral epidermoid carcinoma cell line), Dolnick (1993) cloned a cDNA, which he designated 3-prime rTS, that is antisense to thymidylate synthase (TS, or TYMS; 188350) mRNA. He cloned the full-length cDNA by 5-prime RACE of a squamous cell carcinoma cell line. The mRNA contains 4 possible open reading frames. Dolnick (1993) determined that the 3-prime end of rTS shares 99.5% identity with the antisense strand of the 3-prime untranslated region of TS and contains 2 discontinuities. All of TS exon 7 and part of intron 6 are overlapped by 3-prime rTS. Northern blot analysis of fractionated KB cells and other carcinoma cell lines revealed rTS transcripts of 1.8 and 6.3 kb, as well as several larger mRNA species. The presence and relative abundance of the larger transcripts varied between cell lines, and in vitro translation generated multiple translation products. By screening a KB cell cDNA library for homology with rTS, Dolnick and Black (1996) identified an alternatively spliced transcript, which they called rTS-beta. The rTS-beta transcript has an insertion of 116 nucleotides in its 5-prime region and an altered 3-prime noncoding region that is not complementary to TS mRNA. Dolnick and Black (1996) determined that the original rTS transcript, which they renamed rTS-alpha, encodes a protein with an apparent molecular mass of 41 kD that shares homology with a superfamily of proteins that includes mandelate racemase and muconate-lactonizing enzyme from Pseudomonas putida. The rTS-beta protein was expressed by KB cells and by a colon tumor cell line at an apparent molecular mass of 48 kD.GENE FUNCTION
Dolnick and Black (1996) stated that rTS-alpha was overexpressed at both the RNA and protein level in a leukemic cell line selected for methotrexate resistance and that the overexpressing cell line lost its ability to downregulate TS. Selection for 5-fluorouracil resistance in a colon tumor cell line increased the expression of rTS-alpha and rTS-beta, with highest expression of rTS-beta. Chu and Dolnick (2002) found that rTS-alpha RNA and TS mRNA levels varied inversely when the growth of HEp2 cells progressed from a late-log phase to plateau phase. Transfection and expression of the antisense region of rTS-alpha alone was sufficient to downregulate TS mRNA. Downregulation was also associated with increased site-specific cleavage of TS mRNA.GENE STRUCTURE
Dolnick and Black (1996) determined that the ENOSF1 gene contains at least 8 exons.MAPPING
Dolnick (1993) identified the ENOSF1 gene on the complementary strand of the TYMS gene, which maps to chromosome 18p11.32. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 607427 was added.
Jan. 28, 2016: Protein entry updated
Automatic update: model status changed
Jan. 24, 2016: Protein entry updated
Automatic update: model status changed